1ne9
From Proteopedia
(New page: 200px<br /><applet load="1ne9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ne9, resolution 1.70Å" /> '''Crystal Structure of...) |
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| - | [[Image:1ne9.jpg|left|200px]]<br /><applet load="1ne9" size=" | + | [[Image:1ne9.jpg|left|200px]]<br /><applet load="1ne9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ne9, resolution 1.70Å" /> | caption="1ne9, resolution 1.70Å" /> | ||
'''Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution'''<br /> | '''Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Members of the FemABX protein family are novel therapeutic targets, as | + | Members of the FemABX protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. They catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated tRNA as a substrate. We report here the high-resolution structure of Weissella viridescens L-alanine transferase FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first structure example of a FemABX family member that does not possess a coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the UDP-MurNAc-pentapeptide and a long channel that traverses one of the two domains. Our structural studies bring new insights into the evolution of the FemABX and the related GNAT superfamilies, shed light on the recognition site of the aminoacylated tRNA in Fem proteins, and allowed manual docking of the acceptor end of the alanyl-tRNAAla. |
==About this Structure== | ==About this Structure== | ||
| - | 1NE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NE9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Weissella_viridescens Weissella viridescens] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NE9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Biarrotte-Sorin, S.]] | [[Category: Biarrotte-Sorin, S.]] | ||
[[Category: Delettre, J.]] | [[Category: Delettre, J.]] | ||
| - | [[Category: Maillard, A | + | [[Category: Maillard, A P.]] |
[[Category: Mayer, C.]] | [[Category: Mayer, C.]] | ||
[[Category: Sougakoff, W.]] | [[Category: Sougakoff, W.]] | ||
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[[Category: protein]] | [[Category: protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:10 2008'' |
Revision as of 12:05, 21 February 2008
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Crystal Structure of Weissella viridescens FemX at 1.7 Ang Resolution
Overview
Members of the FemABX protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. They catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated tRNA as a substrate. We report here the high-resolution structure of Weissella viridescens L-alanine transferase FemX and its complex with the UDP-MurNAc-pentapeptide. This is the first structure example of a FemABX family member that does not possess a coiled-coil domain. FemX consists of two structurally equivalent domains, separated by a cleft containing the binding site of the UDP-MurNAc-pentapeptide and a long channel that traverses one of the two domains. Our structural studies bring new insights into the evolution of the FemABX and the related GNAT superfamilies, shed light on the recognition site of the aminoacylated tRNA in Fem proteins, and allowed manual docking of the acceptor end of the alanyl-tRNAAla.
About this Structure
1NE9 is a Single protein structure of sequence from Weissella viridescens with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition., Biarrotte-Sorin S, Maillard AP, Delettre J, Sougakoff W, Arthur M, Mayer C, Structure. 2004 Feb;12(2):257-67. PMID:14962386
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