1nhc
From Proteopedia
(New page: 200px<br /><applet load="1nhc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nhc, resolution 1.70Å" /> '''Structural insights ...) |
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| - | [[Image:1nhc.gif|left|200px]]<br /><applet load="1nhc" size=" | + | [[Image:1nhc.gif|left|200px]]<br /><applet load="1nhc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nhc, resolution 1.70Å" /> | caption="1nhc, resolution 1.70Å" /> | ||
'''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger'''<br /> | '''Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Endopolygalacturonase I is a processive enzyme, while the 60% sequence | + | Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred. |
==About this Structure== | ==About this Structure== | ||
| - | 1NHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with MAN, NAG, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] Full crystallographic information is available from [http:// | + | 1NHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=MAN:'>MAN</scene>, <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polygalacturonase Polygalacturonase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.15 3.2.1.15] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Polygalacturonase]] | [[Category: Polygalacturonase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Benen, J | + | [[Category: Benen, J A.]] |
| - | [[Category: Dijkstra, B | + | [[Category: Dijkstra, B W.]] |
| - | [[Category: Pouderoyen, G | + | [[Category: Pouderoyen, G van.]] |
| - | [[Category: Snijder, H | + | [[Category: Snijder, H J.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: MAN]] | [[Category: MAN]] | ||
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[[Category: beta-helix]] | [[Category: beta-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:10 2008'' |
Revision as of 12:06, 21 February 2008
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Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger
Overview
Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.
About this Structure
1NHC is a Single protein structure of sequence from Aspergillus niger with , , and as ligands. Active as Polygalacturonase, with EC number 3.2.1.15 Full crystallographic information is available from OCA.
Reference
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger., van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW, FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112
Page seeded by OCA on Thu Feb 21 14:06:10 2008
Categories: Aspergillus niger | Polygalacturonase | Single protein | Benen, J A. | Dijkstra, B W. | Pouderoyen, G van. | Snijder, H J. | GOL | MAN | NAG | SO4 | Beta-helix
