1nhv
From Proteopedia
(New page: 200px<br /><applet load="1nhv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nhv, resolution 2.90Å" /> '''Hepatitis C virus RN...) |
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| - | [[Image:1nhv.gif|left|200px]]<br /><applet load="1nhv" size=" | + | [[Image:1nhv.gif|left|200px]]<br /><applet load="1nhv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nhv, resolution 2.90Å" /> | caption="1nhv, resolution 2.90Å" /> | ||
'''Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor'''<br /> | '''Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | X-ray crystal structures of two non-nucleoside analogue inhibitors bound | + | X-ray crystal structures of two non-nucleoside analogue inhibitors bound to hepatitis C virus NS5B RNA-dependent RNA polymerase have been determined to 2.0 and 2.9 A resolution. These noncompetitive inhibitors bind to the same site on the protein, approximately 35 A from the active site. The common features of binding include a large hydrophobic region and two hydrogen bonds between both oxygen atoms of a carboxylate group on the inhibitor and two main chain amide nitrogen atoms of Ser(476) and Tyr(477) on NS5B. The inhibitor-binding site lies at the base of the thumb domain, near its interface with the C-terminal extension of NS5B. The location of this inhibitor-binding site suggests that the binding of these inhibitors interferes with a conformational change essential for the activity of the polymerase. |
==About this Structure== | ==About this Structure== | ||
| - | 1NHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with 154 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | + | 1NHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus] with <scene name='pdbligand=154:'>154</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NHV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alaoui-Ismaili, M | + | [[Category: Alaoui-Ismaili, M H.]] |
[[Category: Bedard, J.]] | [[Category: Bedard, J.]] | ||
| - | [[Category: Bethell, R | + | [[Category: Bethell, R C.]] |
[[Category: Chan, L.]] | [[Category: Chan, L.]] | ||
| - | [[Category: Cherney, M | + | [[Category: Cherney, M M.]] |
| - | [[Category: James, M | + | [[Category: James, M N.G.]] |
[[Category: Morin, N.]] | [[Category: Morin, N.]] | ||
| - | [[Category: Ng, K | + | [[Category: Ng, K K.S.]] |
[[Category: Nguyen-Ba, N.]] | [[Category: Nguyen-Ba, N.]] | ||
[[Category: Wang, M.]] | [[Category: Wang, M.]] | ||
| - | [[Category: Yannopoulos, C | + | [[Category: Yannopoulos, C G.]] |
[[Category: 154]] | [[Category: 154]] | ||
[[Category: hepatitis c; rna polymerase; enzyme inhibition]] | [[Category: hepatitis c; rna polymerase; enzyme inhibition]] | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:15 2008'' |
Revision as of 12:06, 21 February 2008
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Hepatitis C virus RNA polymerase in complex with non-nucleoside analogue inhibitor
Overview
X-ray crystal structures of two non-nucleoside analogue inhibitors bound to hepatitis C virus NS5B RNA-dependent RNA polymerase have been determined to 2.0 and 2.9 A resolution. These noncompetitive inhibitors bind to the same site on the protein, approximately 35 A from the active site. The common features of binding include a large hydrophobic region and two hydrogen bonds between both oxygen atoms of a carboxylate group on the inhibitor and two main chain amide nitrogen atoms of Ser(476) and Tyr(477) on NS5B. The inhibitor-binding site lies at the base of the thumb domain, near its interface with the C-terminal extension of NS5B. The location of this inhibitor-binding site suggests that the binding of these inhibitors interferes with a conformational change essential for the activity of the polymerase.
About this Structure
1NHV is a Single protein structure of sequence from Hepatitis c virus with as ligand. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Non-nucleoside analogue inhibitors bind to an allosteric site on HCV NS5B polymerase. Crystal structures and mechanism of inhibition., Wang M, Ng KK, Cherney MM, Chan L, Yannopoulos CG, Bedard J, Morin N, Nguyen-Ba N, Alaoui-Ismaili MH, Bethell RC, James MN, J Biol Chem. 2003 Mar 14;278(11):9489-95. Epub 2002 Dec 30. PMID:12509436
Page seeded by OCA on Thu Feb 21 14:06:15 2008
Categories: Hepatitis c virus | RNA-directed RNA polymerase | Single protein | Alaoui-Ismaili, M H. | Bedard, J. | Bethell, R C. | Chan, L. | Cherney, M M. | James, M N.G. | Morin, N. | Ng, K K.S. | Nguyen-Ba, N. | Wang, M. | Yannopoulos, C G. | 154 | Hepatitis c; rna polymerase; enzyme inhibition | X-ray crystallography
