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1nio
From Proteopedia
(New page: 200px<br /><applet load="1nio" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nio, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1nio.gif|left|200px]]<br /><applet load="1nio" size=" | + | [[Image:1nio.gif|left|200px]]<br /><applet load="1nio" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nio, resolution 2.00Å" /> | caption="1nio, resolution 2.00Å" /> | ||
'''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution'''<br /> | '''Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of beta-luffin at 2.0 A resolution was solved by the | + | The crystal structure of beta-luffin at 2.0 A resolution was solved by the molecular-replacement method using polyalanyl trichosanthin as the search model. The structure was refined with CNS1.1, giving R(work) = 0.162 and R(free) = 0.204. The r.m.s.d.s of the bond lengths and bond angles are 0.008 A and 1.3 degrees, respectively. The overall structure is similar to those of other type I RIPs. Three N-acetylglucosamine (Nag) molecules are linked to residues Asn2, Asn78 and Asn85 of the protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1NIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http:// | + | 1NIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Luffa_aegyptiaca Luffa aegyptiaca] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NIO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: ribosome inactivating protein(rip)]] | [[Category: ribosome inactivating protein(rip)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:34 2008'' |
Revision as of 12:06, 21 February 2008
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Crystal structure of beta-luffin, a ribosome inactivating protein at 2.0A resolution
Overview
The crystal structure of beta-luffin at 2.0 A resolution was solved by the molecular-replacement method using polyalanyl trichosanthin as the search model. The structure was refined with CNS1.1, giving R(work) = 0.162 and R(free) = 0.204. The r.m.s.d.s of the bond lengths and bond angles are 0.008 A and 1.3 degrees, respectively. The overall structure is similar to those of other type I RIPs. Three N-acetylglucosamine (Nag) molecules are linked to residues Asn2, Asn78 and Asn85 of the protein.
About this Structure
1NIO is a Single protein structure of sequence from Luffa aegyptiaca with as ligand. Active as rRNA N-glycosylase, with EC number 3.2.2.22 Full crystallographic information is available from OCA.
Reference
Crystal structure of beta-luffin, a ribosome-inactivating protein, at 2.0 A resolution., Ma QJ, Li JH, Li HG, Wu S, Dong YC, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1366-70. Epub 2003, Jul 23. PMID:12876337
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