1npo
From Proteopedia
(New page: 200px<br /><applet load="1npo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1npo, resolution 3.0Å" /> '''BOVINE NEUROPHYSIN II...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1npo.gif|left|200px]]<br /><applet load="1npo" size=" | + | [[Image:1npo.gif|left|200px]]<br /><applet load="1npo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1npo, resolution 3.0Å" /> | caption="1npo, resolution 3.0Å" /> | ||
'''BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN'''<br /> | '''BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The first crystal structure of the pituitary hormone oxytocin complexed | + | The first crystal structure of the pituitary hormone oxytocin complexed with its carrier protein neurophysin has been determined and refined to 3.0 A resolution. The hormone-binding site is located at the end of a 3(10)-helix and involves residues from both domains of each monomer. Hormone residues Tyr 2, which is buried deep in the binding pocket, and Cys 1 have been confirmed as the key residues involved in neurophysin-hormone recognition. We have compared the bound oxytocin observed in the neurophysin-oxytocin complex, the X-ray structures of unbound oxytocin analogues and the NMR-derived structure for bound oxytocin. We find that while our structure is in agreement with the previous crystallographic findings, it differs from the NMR result with regard to how Tyr 2 of the hormone is recognized by neurophysin. |
==About this Structure== | ==About this Structure== | ||
| - | 1NPO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | + | 1NPO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPO OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Rose, J | + | [[Category: Rose, J P.]] |
| - | [[Category: Wang, B | + | [[Category: Wang, B C.]] |
[[Category: complex (hormone transport/hormone)]] | [[Category: complex (hormone transport/hormone)]] | ||
[[Category: hypothalamus]] | [[Category: hypothalamus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:43 2008'' |
Revision as of 12:08, 21 February 2008
|
BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN
Overview
The first crystal structure of the pituitary hormone oxytocin complexed with its carrier protein neurophysin has been determined and refined to 3.0 A resolution. The hormone-binding site is located at the end of a 3(10)-helix and involves residues from both domains of each monomer. Hormone residues Tyr 2, which is buried deep in the binding pocket, and Cys 1 have been confirmed as the key residues involved in neurophysin-hormone recognition. We have compared the bound oxytocin observed in the neurophysin-oxytocin complex, the X-ray structures of unbound oxytocin analogues and the NMR-derived structure for bound oxytocin. We find that while our structure is in agreement with the previous crystallographic findings, it differs from the NMR result with regard to how Tyr 2 of the hormone is recognized by neurophysin.
About this Structure
1NPO is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the neurophysin-oxytocin complex., Rose JP, Wu CK, Hsiao CD, Breslow E, Wang BC, Nat Struct Biol. 1996 Feb;3(2):163-9. PMID:8564543
Page seeded by OCA on Thu Feb 21 14:08:43 2008
