1nr2
From Proteopedia
(New page: 200px<br /> <applet load="1nr2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nr2, resolution 2.18Å" /> '''High resolution cry...) |
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| - | [[Image:1nr2.gif|left|200px]]<br /> | + | [[Image:1nr2.gif|left|200px]]<br /><applet load="1nr2" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1nr2, resolution 2.18Å" /> | caption="1nr2, resolution 2.18Å" /> | ||
'''High resolution crystal structures of thymus and activation-regulated chemokine'''<br /> | '''High resolution crystal structures of thymus and activation-regulated chemokine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Thymus and activation-regulated chemokine (TARC) is a CC chemokine that is | + | Thymus and activation-regulated chemokine (TARC) is a CC chemokine that is mainly expressed in the thymus. TARC interacts primarily with the CCR4 receptor and to a lesser extent with the CCR8 receptor. The structures of TARC have been solved by molecular replacement in two space groups, triclinic (P1) and tetragonal (P4(1)), and refined to resolutions of 1.72 and 2.1 A, respectively, with R factors of 19.8% (R(free) = 24.1%) and 19.8% (R(free) = 27.7%), respectively. The search model originated from the crystal structure of another chemokine, RANTES, and proved to be only modestly similar to the refined structure of TARC. Whereas the tetragonal structure was easily solved using the program AMoRe, solution of the triclinic structure proved to be quite challenging and was obtained by combining the results from four different molecular-replacement programs (AMoRe, CNS, BEAST and EPMR), with subsequent extension of the gathered information. The tertiary structure of TARC is similar to that of other CC chemokines, with a three-stranded antiparallel beta-sheet flanked by a C-terminal helix. Both quaternary structures consist of dimers, which in the triclinic crystals pack further into tetramers. The TARC dimers are similar to those observed previously in the crystal structures of both MCP-1 and RANTES. |
==About this Structure== | ==About this Structure== | ||
| - | 1NR2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1NR2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NR2 OCA]. |
==Reference== | ==Reference== | ||
Structures of thymus and activation-regulated chemokine (TARC)., Asojo OA, Boulegue C, Hoover DM, Lu W, Lubkowski J, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1165-73. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832759 12832759] | Structures of thymus and activation-regulated chemokine (TARC)., Asojo OA, Boulegue C, Hoover DM, Lu W, Lubkowski J, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1165-73. Epub 2003, Jun 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12832759 12832759] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Asojo, O | + | [[Category: Asojo, O A.]] |
[[Category: Boulegue, C.]] | [[Category: Boulegue, C.]] | ||
| - | [[Category: Hoover, D | + | [[Category: Hoover, D M.]] |
[[Category: Lu, W.]] | [[Category: Lu, W.]] | ||
[[Category: Lubkowski, J.]] | [[Category: Lubkowski, J.]] | ||
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[[Category: tarc]] | [[Category: tarc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:06 2008'' |
Revision as of 12:09, 21 February 2008
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High resolution crystal structures of thymus and activation-regulated chemokine
Overview
Thymus and activation-regulated chemokine (TARC) is a CC chemokine that is mainly expressed in the thymus. TARC interacts primarily with the CCR4 receptor and to a lesser extent with the CCR8 receptor. The structures of TARC have been solved by molecular replacement in two space groups, triclinic (P1) and tetragonal (P4(1)), and refined to resolutions of 1.72 and 2.1 A, respectively, with R factors of 19.8% (R(free) = 24.1%) and 19.8% (R(free) = 27.7%), respectively. The search model originated from the crystal structure of another chemokine, RANTES, and proved to be only modestly similar to the refined structure of TARC. Whereas the tetragonal structure was easily solved using the program AMoRe, solution of the triclinic structure proved to be quite challenging and was obtained by combining the results from four different molecular-replacement programs (AMoRe, CNS, BEAST and EPMR), with subsequent extension of the gathered information. The tertiary structure of TARC is similar to that of other CC chemokines, with a three-stranded antiparallel beta-sheet flanked by a C-terminal helix. Both quaternary structures consist of dimers, which in the triclinic crystals pack further into tetramers. The TARC dimers are similar to those observed previously in the crystal structures of both MCP-1 and RANTES.
About this Structure
1NR2 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Structures of thymus and activation-regulated chemokine (TARC)., Asojo OA, Boulegue C, Hoover DM, Lu W, Lubkowski J, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1165-73. Epub 2003, Jun 27. PMID:12832759
Page seeded by OCA on Thu Feb 21 14:09:06 2008
Categories: Single protein | Asojo, O A. | Boulegue, C. | Hoover, D M. | Lu, W. | Lubkowski, J. | Cc-chemokine | Chemokine | Chemotaxis | Cytokine | Tarc
