1nrg
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the | + | Pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the synthesis of pyridoxal 5'-phosphate. The cDNA for the human enzyme has been cloned and expressed in Escherichia coli. The purified human enzyme is a homodimer that exhibits a low catalytic rate constant of approximately 0.2 sec(-1) and K(m) values in the low micromolar range for both pyridoxine 5'phosphate and pyridoxamine 5'-phosphate. Pyridoxal 5'-phosphate is an effective product inhibitor. The three-dimensional fold of the human enzyme is very similar to those of the E. coli and yeast enzymes. The human and E. coli enzymes share 39% sequence identity, but the binding sites for the tightly bound FMN and substrate are highly conserved. As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit. |
==Disease== | ==Disease== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ko, T | + | [[Category: Ko, T P.]] |
| - | [[Category: Musayev, F | + | [[Category: Musayev, F N.]] |
| - | [[Category: Safo, M | + | [[Category: Safo, M K.]] |
| - | [[Category: Salvo, M | + | [[Category: Salvo, M L.di.]] |
[[Category: Schirch, V.]] | [[Category: Schirch, V.]] | ||
[[Category: BME]] | [[Category: BME]] | ||
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[[Category: pyridoxine-5'-phosphate]] | [[Category: pyridoxine-5'-phosphate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:19 2008'' |
Revision as of 12:09, 21 February 2008
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Structure and Properties of Recombinant Human Pyridoxine-5'-Phosphate Oxidase
Contents |
Overview
Pyridoxine 5'-phosphate oxidase catalyzes the terminal step in the synthesis of pyridoxal 5'-phosphate. The cDNA for the human enzyme has been cloned and expressed in Escherichia coli. The purified human enzyme is a homodimer that exhibits a low catalytic rate constant of approximately 0.2 sec(-1) and K(m) values in the low micromolar range for both pyridoxine 5'phosphate and pyridoxamine 5'-phosphate. Pyridoxal 5'-phosphate is an effective product inhibitor. The three-dimensional fold of the human enzyme is very similar to those of the E. coli and yeast enzymes. The human and E. coli enzymes share 39% sequence identity, but the binding sites for the tightly bound FMN and substrate are highly conserved. As observed with the E. coli enzyme, the human enzyme binds one molecule of pyridoxal 5'-phosphate tightly on each subunit.
Disease
Known disease associated with this structure: Pyridoxamine 5 -phosphate oxidase deficiency OMIM:[603287]
About this Structure
1NRG is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structure and properties of recombinant human pyridoxine 5'-phosphate oxidase., Musayev FN, Di Salvo ML, Ko TP, Schirch V, Safo MK, Protein Sci. 2003 Jul;12(7):1455-63. PMID:12824491
Page seeded by OCA on Thu Feb 21 14:09:19 2008
Categories: Homo sapiens | Single protein | Ko, T P. | Musayev, F N. | Safo, M K. | Salvo, M L.di. | Schirch, V. | BME | FMN | PLP | PO4 | Fmn | Oxidase | Plp | Pyridoxine-5'-phosphate
