1nt0
From Proteopedia
(New page: 200px<br /><applet load="1nt0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nt0, resolution 2.70Å" /> '''Crystal structure of...) |
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| - | [[Image:1nt0.gif|left|200px]]<br /><applet load="1nt0" size=" | + | [[Image:1nt0.gif|left|200px]]<br /><applet load="1nt0" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nt0, resolution 2.70Å" /> | caption="1nt0, resolution 2.70Å" /> | ||
'''Crystal structure of the CUB1-EGF-CUB2 region of MASP2'''<br /> | '''Crystal structure of the CUB1-EGF-CUB2 region of MASP2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Serum mannose-binding proteins (MBPs) are C-type lectins that recognize | + | Serum mannose-binding proteins (MBPs) are C-type lectins that recognize cell surface carbohydrate structures on pathogens, and trigger killing of these targets by activating the complement pathway. MBPs circulate as a complex with MBP-associated serine proteases (MASPs), which become activated upon engagement of a target cell surface. The minimal functional unit for complement activation is a MASP homodimer bound to two MBP trimeric subunits. MASPs have a modular structure consisting of an N-terminal CUB domain, a Ca(2+)-binding EGF-like domain, a second CUB domain, two complement control protein modules and a C-terminal serine protease domain. The CUB1-EGF-CUB2 region mediates homodimerization and binding to MBP. The crystal structure of the MASP-2 CUB1-EGF-CUB2 dimer reveals an elongated structure with a prominent concave surface that is proposed to be the MBP-binding site. A model of the full six-domain structure and its interaction with MBPs suggests mechanisms by which binding to a target cell transmits conformational changes from MBP to MASP that allow activation of its protease activity. |
==About this Structure== | ==About this Structure== | ||
| - | 1NT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAG, CA and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1NT0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT0 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Davies, J | + | [[Category: Davies, J M.]] |
[[Category: Drickamer, K.]] | [[Category: Drickamer, K.]] | ||
[[Category: Feinberg, H.]] | [[Category: Feinberg, H.]] | ||
| - | [[Category: Uitdehaag, J | + | [[Category: Uitdehaag, J C.M.]] |
[[Category: Wallis, R.]] | [[Category: Wallis, R.]] | ||
| - | [[Category: Weis, W | + | [[Category: Weis, W I.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: EDO]] | [[Category: EDO]] | ||
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[[Category: masp]] | [[Category: masp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:38 2008'' |
Revision as of 12:09, 21 February 2008
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Crystal structure of the CUB1-EGF-CUB2 region of MASP2
Overview
Serum mannose-binding proteins (MBPs) are C-type lectins that recognize cell surface carbohydrate structures on pathogens, and trigger killing of these targets by activating the complement pathway. MBPs circulate as a complex with MBP-associated serine proteases (MASPs), which become activated upon engagement of a target cell surface. The minimal functional unit for complement activation is a MASP homodimer bound to two MBP trimeric subunits. MASPs have a modular structure consisting of an N-terminal CUB domain, a Ca(2+)-binding EGF-like domain, a second CUB domain, two complement control protein modules and a C-terminal serine protease domain. The CUB1-EGF-CUB2 region mediates homodimerization and binding to MBP. The crystal structure of the MASP-2 CUB1-EGF-CUB2 dimer reveals an elongated structure with a prominent concave surface that is proposed to be the MBP-binding site. A model of the full six-domain structure and its interaction with MBPs suggests mechanisms by which binding to a target cell transmits conformational changes from MBP to MASP that allow activation of its protease activity.
About this Structure
1NT0 is a Single protein structure of sequence from Rattus norvegicus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2., Feinberg H, Uitdehaag JC, Davies JM, Wallis R, Drickamer K, Weis WI, EMBO J. 2003 May 15;22(10):2348-59. PMID:12743029
Page seeded by OCA on Thu Feb 21 14:09:38 2008
Categories: Rattus norvegicus | Single protein | Davies, J M. | Drickamer, K. | Feinberg, H. | Uitdehaag, J C.M. | Wallis, R. | Weis, W I. | CA | EDO | NAG | Cub domain | Egf like domain. | Mannose-binding protein | Masp
