1nsw
From Proteopedia
(New page: 200px<br /><applet load="1nsw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nsw, resolution 1.90Å" /> '''The Crystal Structur...) |
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| - | [[Image:1nsw.gif|left|200px]]<br /><applet load="1nsw" size=" | + | [[Image:1nsw.gif|left|200px]]<br /><applet load="1nsw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nsw, resolution 1.90Å" /> | caption="1nsw, resolution 1.90Å" /> | ||
'''The Crystal Structure of the K18G Mutant of the thioredoxin from Alicyclobacillus acidocaldarius'''<br /> | '''The Crystal Structure of the K18G Mutant of the thioredoxin from Alicyclobacillus acidocaldarius'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report a crystallographic and computational analysis of two mutant | + | We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein. |
==About this Structure== | ==About this Structure== | ||
| - | 1NSW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]. Active as [http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] Full crystallographic information is available from [http:// | + | 1NSW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alicyclobacillus_acidocaldarius Alicyclobacillus acidocaldarius]. Active as [http://en.wikipedia.org/wiki/Thioredoxin-disulfide_reductase Thioredoxin-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.9 1.8.1.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NSW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pedone, E.]] | [[Category: Pedone, E.]] | ||
[[Category: Saviano, M.]] | [[Category: Saviano, M.]] | ||
| - | [[Category: Simone, G | + | [[Category: Simone, G De.]] |
[[Category: thermostability]] | [[Category: thermostability]] | ||
[[Category: thioredoxin]] | [[Category: thioredoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:43 2008'' |
Revision as of 12:09, 21 February 2008
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The Crystal Structure of the K18G Mutant of the thioredoxin from Alicyclobacillus acidocaldarius
Overview
We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.
About this Structure
1NSW is a Single protein structure of sequence from Alicyclobacillus acidocaldarius. Active as Thioredoxin-disulfide reductase, with EC number 1.8.1.9 Full crystallographic information is available from OCA.
Reference
An integrated structural and computational study of the thermostability of two thioredoxin mutants from Alicyclobacillus acidocaldarius., Bartolucci S, De Simone G, Galdiero S, Improta R, Menchise V, Pedone C, Pedone E, Saviano M, J Bacteriol. 2003 Jul;185(14):4285-9. PMID:12837806
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