1ntv
From Proteopedia
(New page: 200px<br /><applet load="1ntv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ntv, resolution 1.50Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ntv.gif|left|200px]]<br /><applet load="1ntv" size=" | + | [[Image:1ntv.gif|left|200px]]<br /><applet load="1ntv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ntv, resolution 1.50Å" /> | caption="1ntv, resolution 1.50Å" /> | ||
'''Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex'''<br /> | '''Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Formation of the mammalian six-layered neocortex depends on a signaling | + | Formation of the mammalian six-layered neocortex depends on a signaling pathway that involves Reelin, the very low-density lipoprotein receptor, the apolipoprotein E receptor-2 (ApoER2), and the adaptor protein Disabled-1 (Dab1). The 1.5 A crystal structure of a complex between the Dab1 phosphotyrosine binding (PTB) domain and a 14-residue peptide from the ApoER2 tail explains the unusual preference of Dab1 for unphosphorylated tyrosine within the NPxY motif of the peptide. Crystals of the complex soaked with the phosphoinositide PI-4,5P(2) (PI) show that PI binds to conserved basic residues on the PTB domain opposite the peptide binding groove. This finding explains how the Dab1 PTB domain can simultaneously bind PI and the ApoER2 tail. Recruitment of the Dab1 PTB domain to PI-rich regions of the plasma membrane may facilitate association with the Reelin receptor cytoplasmic tails to transduce a critical positional cue to migrating neurons. |
==About this Structure== | ==About this Structure== | ||
| - | 1NTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1NTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTV OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blacklow, S | + | [[Category: Blacklow, S C.]] |
| - | [[Category: Eck, M | + | [[Category: Eck, M J.]] |
[[Category: Herz, J.]] | [[Category: Herz, J.]] | ||
[[Category: Jeon, H.]] | [[Category: Jeon, H.]] | ||
| - | [[Category: Song, H | + | [[Category: Song, H K.]] |
| - | [[Category: Stolt, P | + | [[Category: Stolt, P C.]] |
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: beta-sandwich]] | [[Category: beta-sandwich]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:56 2008'' |
Revision as of 12:09, 21 February 2008
|
Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex
Overview
Formation of the mammalian six-layered neocortex depends on a signaling pathway that involves Reelin, the very low-density lipoprotein receptor, the apolipoprotein E receptor-2 (ApoER2), and the adaptor protein Disabled-1 (Dab1). The 1.5 A crystal structure of a complex between the Dab1 phosphotyrosine binding (PTB) domain and a 14-residue peptide from the ApoER2 tail explains the unusual preference of Dab1 for unphosphorylated tyrosine within the NPxY motif of the peptide. Crystals of the complex soaked with the phosphoinositide PI-4,5P(2) (PI) show that PI binds to conserved basic residues on the PTB domain opposite the peptide binding groove. This finding explains how the Dab1 PTB domain can simultaneously bind PI and the ApoER2 tail. Recruitment of the Dab1 PTB domain to PI-rich regions of the plasma membrane may facilitate association with the Reelin receptor cytoplasmic tails to transduce a critical positional cue to migrating neurons.
About this Structure
1NTV is a Single protein structure of sequence from Mus musculus with as ligand. Full crystallographic information is available from OCA.
Reference
Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes., Stolt PC, Jeon H, Song HK, Herz J, Eck MJ, Blacklow SC, Structure. 2003 May;11(5):569-79. PMID:12737822
Page seeded by OCA on Thu Feb 21 14:09:56 2008
Categories: Mus musculus | Single protein | Blacklow, S C. | Eck, M J. | Herz, J. | Jeon, H. | Song, H K. | Stolt, P C. | PO4 | Beta-sandwich
