1ntx
From Proteopedia
(New page: 200px<br /><applet load="1ntx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ntx" /> '''SECONDARY STRUCTURE DETERMINATION FOR ALPHA-...) |
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| - | [[Image:1ntx.gif|left|200px]]<br /><applet load="1ntx" size=" | + | [[Image:1ntx.gif|left|200px]]<br /><applet load="1ntx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ntx" /> | caption="1ntx" /> | ||
'''SECONDARY STRUCTURE DETERMINATION FOR ALPHA-NEUROTOXIN FROM DENDROASPIS POLYLEPIS POLYLEPIS BASED ON SEQUENCE SPECIFIC PROTON NUCLEAR MAGNETIC RESONANCE ASSIGNMENTS'''<br /> | '''SECONDARY STRUCTURE DETERMINATION FOR ALPHA-NEUROTOXIN FROM DENDROASPIS POLYLEPIS POLYLEPIS BASED ON SEQUENCE SPECIFIC PROTON NUCLEAR MAGNETIC RESONANCE ASSIGNMENTS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sequence-specific assignments are presented for the polypeptide backbone | + | Sequence-specific assignments are presented for the polypeptide backbone protons and a majority of the amino-acid-side-chain protons of alpha-neurotoxin from Dendroaspis polylepis polylepis, and individual amide proton-exchange rates with the solvent are reported. The secondary structure and the hydrogen-bonding patterns in the regular secondary structure elements are deduced from nuclear Overhauser effects and the sequence locations of the slowly exchanging amide protons. The molecule includes a three-stranded antiparallel beta-sheet, and there are indications that two additional short chain segments are arranged in an antiparallel beta-sheet. These structural elements are similar, but not identical, to either the secondary structure reported for erabutoxin b in single crystals, or the solution structure of cytotoxin CTXIIb from Naja mossambica mossambica. |
==About this Structure== | ==About this Structure== | ||
| - | 1NTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full crystallographic information is available from [http:// | + | 1NTX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dendroaspis_polylepis_polylepis Dendroaspis polylepis polylepis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dendroaspis polylepis polylepis]] | [[Category: Dendroaspis polylepis polylepis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brown, L | + | [[Category: Brown, L R.]] |
[[Category: Wuthrich, K.]] | [[Category: Wuthrich, K.]] | ||
[[Category: neurotoxin]] | [[Category: neurotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:56 2008'' |
Revision as of 12:09, 21 February 2008
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SECONDARY STRUCTURE DETERMINATION FOR ALPHA-NEUROTOXIN FROM DENDROASPIS POLYLEPIS POLYLEPIS BASED ON SEQUENCE SPECIFIC PROTON NUCLEAR MAGNETIC RESONANCE ASSIGNMENTS
Overview
Sequence-specific assignments are presented for the polypeptide backbone protons and a majority of the amino-acid-side-chain protons of alpha-neurotoxin from Dendroaspis polylepis polylepis, and individual amide proton-exchange rates with the solvent are reported. The secondary structure and the hydrogen-bonding patterns in the regular secondary structure elements are deduced from nuclear Overhauser effects and the sequence locations of the slowly exchanging amide protons. The molecule includes a three-stranded antiparallel beta-sheet, and there are indications that two additional short chain segments are arranged in an antiparallel beta-sheet. These structural elements are similar, but not identical, to either the secondary structure reported for erabutoxin b in single crystals, or the solution structure of cytotoxin CTXIIb from Naja mossambica mossambica.
About this Structure
1NTX is a Single protein structure of sequence from Dendroaspis polylepis polylepis. Full crystallographic information is available from OCA.
Reference
Secondary structure determination for alpha-neurotoxin from Dendroaspis polylepis polylepis based on sequence-specific 1H-nuclear-magnetic-resonance assignments., Labhardt AM, Hunziker-Kwik EH, Wuthrich K, Eur J Biochem. 1988 Nov 1;177(2):295-305. PMID:2847926
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