1nwa
From Proteopedia
(New page: 200px<br /><applet load="1nwa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nwa, resolution 1.5Å" /> '''Structure of Mycobact...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1nwa.gif|left|200px]]<br /><applet load="1nwa" size=" | + | [[Image:1nwa.gif|left|200px]]<br /><applet load="1nwa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nwa, resolution 1.5Å" /> | caption="1nwa, resolution 1.5Å" /> | ||
'''Structure of Mycobacterium tuberculosis Methionine Sulfoxide Reductase A in Complex with Protein-bound Methionine'''<br /> | '''Structure of Mycobacterium tuberculosis Methionine Sulfoxide Reductase A in Complex with Protein-bound Methionine'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Peptide methionine sulfoxide reductase (MsrA) repairs oxidative damage to | + | Peptide methionine sulfoxide reductase (MsrA) repairs oxidative damage to methionine residues arising from reactive oxygen species and reactive nitrogen intermediates. MsrA activity is found in a wide variety of organisms, and it is implicated as one of the primary defenses against oxidative stress. Disruption of the gene encoding MsrA in several pathogenic bacteria responsible for infections in humans results in the loss of their ability to colonize host cells. Here, we present the X-ray crystal structure of MsrA from the pathogenic bacterium Mycobacterium tuberculosis refined to 1.5 A resolution. In contrast to the three catalytic cysteine residues found in previously characterized MsrA structures, M. tuberculosis MsrA represents a class containing only two functional cysteine residues. The structure reveals a methionine residue of one MsrA molecule bound at the active site of a neighboring molecule in the crystal lattice and thus serves as an excellent model for protein-bound methionine sulfoxide recognition and repair. |
==About this Structure== | ==About this Structure== | ||
| - | 1NWA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Peptide-methionine-(S)-S-oxide_reductase Peptide-methionine-(S)-S-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.11 1.8.4.11] Full crystallographic information is available from [http:// | + | 1NWA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Active as [http://en.wikipedia.org/wiki/Peptide-methionine-(S)-S-oxide_reductase Peptide-methionine-(S)-S-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.4.11 1.8.4.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWA OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Dhandayuthapani, S.]] | [[Category: Dhandayuthapani, S.]] | ||
| - | [[Category: Hart, P | + | [[Category: Hart, P J.]] |
| - | [[Category: Jr., D | + | [[Category: Jr., D M.Benglis.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
| - | [[Category: Taylor, A | + | [[Category: Taylor, A B.]] |
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
[[Category: peptide methionine sulfoxide reductase]] | [[Category: peptide methionine sulfoxide reductase]] | ||
| Line 28: | Line 28: | ||
[[Category: tbsgc]] | [[Category: tbsgc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:47 2008'' |
Revision as of 12:10, 21 February 2008
|
Structure of Mycobacterium tuberculosis Methionine Sulfoxide Reductase A in Complex with Protein-bound Methionine
Overview
Peptide methionine sulfoxide reductase (MsrA) repairs oxidative damage to methionine residues arising from reactive oxygen species and reactive nitrogen intermediates. MsrA activity is found in a wide variety of organisms, and it is implicated as one of the primary defenses against oxidative stress. Disruption of the gene encoding MsrA in several pathogenic bacteria responsible for infections in humans results in the loss of their ability to colonize host cells. Here, we present the X-ray crystal structure of MsrA from the pathogenic bacterium Mycobacterium tuberculosis refined to 1.5 A resolution. In contrast to the three catalytic cysteine residues found in previously characterized MsrA structures, M. tuberculosis MsrA represents a class containing only two functional cysteine residues. The structure reveals a methionine residue of one MsrA molecule bound at the active site of a neighboring molecule in the crystal lattice and thus serves as an excellent model for protein-bound methionine sulfoxide recognition and repair.
About this Structure
1NWA is a Single protein structure of sequence from Mycobacterium tuberculosis. Active as Peptide-methionine-(S)-S-oxide reductase, with EC number 1.8.4.11 Full crystallographic information is available from OCA.
Reference
Structure of Mycobacterium tuberculosis methionine sulfoxide reductase A in complex with protein-bound methionine., Taylor AB, Benglis DM Jr, Dhandayuthapani S, Hart PJ, J Bacteriol. 2003 Jul;185(14):4119-26. PMID:12837786
Page seeded by OCA on Thu Feb 21 14:10:47 2008
Categories: Mycobacterium tuberculosis | Peptide-methionine-(S)-S-oxide reductase | Single protein | Dhandayuthapani, S. | Hart, P J. | Jr., D M.Benglis. | TBSGC, TB Structural Genomics Consortium. | Taylor, A B. | Oxidoreductase | Peptide methionine sulfoxide reductase | Product complex | Protein structure initiative | Psi | Structural genomics | Tb structural genomics consortium | Tbsgc
