1nwm
From Proteopedia
(New page: 200px<br /> <applet load="1nwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nwm, resolution 2.40Å" /> '''GAT domain of human...) |
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| - | [[Image:1nwm.gif|left|200px]]<br /> | + | [[Image:1nwm.gif|left|200px]]<br /><applet load="1nwm" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1nwm" size=" | + | |
caption="1nwm, resolution 2.40Å" /> | caption="1nwm, resolution 2.40Å" /> | ||
'''GAT domain of human GGA1'''<br /> | '''GAT domain of human GGA1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Golgi-associated, gamma-adaptin homologous, ADP-ribosylation factor | + | The Golgi-associated, gamma-adaptin homologous, ADP-ribosylation factor (ARF)-interacting proteins (GGAs) are adaptors that sort receptors from the trans-Golgi network into the endosomallysosomal pathway. The GGAs and TOM1 (GAT) domains of the GGAs are responsible for their ARF-dependent localization. The 2.4-A crystal structure of the GAT domain of human GGA1 reveals a three-helix bundle, with a long N-terminal helical extension that is not conserved in GAT domains that do not bind ARF. The ARF binding site is located in the N-terminal extension and is separate from the core three-helix bundle. An unanticipated structural similarity to the N-terminal domain of syntaxin 1a was discovered, comprising the entire three-helix bundle. A conserved binding site on helices 2 and 3 of the GAT domain three-helix bundle is predicted to interact with coiled-coil-containing proteins. We propose that the GAT domain is descended from the same ancestor as the syntaxin 1a N-terminal domain, and that both protein families share a common function in binding coiled-coil domain proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1NWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1NWM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hurley, J | + | [[Category: Hurley, J H.]] |
[[Category: Misra, S.]] | [[Category: Misra, S.]] | ||
| - | [[Category: Saidi, L | + | [[Category: Saidi, L F.]] |
[[Category: Suer, S.]] | [[Category: Suer, S.]] | ||
[[Category: three-alpha helical bundle]] | [[Category: three-alpha helical bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:55 2008'' |
Revision as of 12:11, 21 February 2008
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GAT domain of human GGA1
Overview
The Golgi-associated, gamma-adaptin homologous, ADP-ribosylation factor (ARF)-interacting proteins (GGAs) are adaptors that sort receptors from the trans-Golgi network into the endosomallysosomal pathway. The GGAs and TOM1 (GAT) domains of the GGAs are responsible for their ARF-dependent localization. The 2.4-A crystal structure of the GAT domain of human GGA1 reveals a three-helix bundle, with a long N-terminal helical extension that is not conserved in GAT domains that do not bind ARF. The ARF binding site is located in the N-terminal extension and is separate from the core three-helix bundle. An unanticipated structural similarity to the N-terminal domain of syntaxin 1a was discovered, comprising the entire three-helix bundle. A conserved binding site on helices 2 and 3 of the GAT domain three-helix bundle is predicted to interact with coiled-coil-containing proteins. We propose that the GAT domain is descended from the same ancestor as the syntaxin 1a N-terminal domain, and that both protein families share a common function in binding coiled-coil domain proteins.
About this Structure
1NWM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the GAT domain of human GGA1: a syntaxin amino-terminal domain fold in an endosomal trafficking adaptor., Suer S, Misra S, Saidi LF, Hurley JH, Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4451-6. Epub 2003 Mar 31. PMID:12668765
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