1nz6
From Proteopedia
(New page: 200px<br /><applet load="1nz6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nz6, resolution 2.5Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1nz6.jpg|left|200px]]<br /><applet load="1nz6" size=" | + | [[Image:1nz6.jpg|left|200px]]<br /><applet load="1nz6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nz6, resolution 2.5Å" /> | caption="1nz6, resolution 2.5Å" /> | ||
'''Crystal Structure of Auxilin J-Domain'''<br /> | '''Crystal Structure of Auxilin J-Domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | J-domains are widespread protein interaction modules involved in | + | J-domains are widespread protein interaction modules involved in recruiting and stimulating the activity of Hsp70 family chaperones. We have determined the crystal structure of the J-domain of auxilin, a protein which is involved in uncoating clathrin-coated vesicles. Comparison to the known structures of J-domains from four other proteins reveals that the auxilin J-domain is the most divergent of all J-domain structures described to date. In addition to the canonical J-domain features described previously, the auxilin J-domain contains an extra N-terminal helix and a long loop inserted between helices I and II. The latter loop extends the positively charged surface which forms the Hsc70 binding site, and is shown by directed mutagenesis and surface plasmon resonance to contain side chains important for binding to Hsc70. |
==About this Structure== | ==About this Structure== | ||
| - | 1NZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http:// | + | 1NZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZ6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hart, P | + | [[Category: Hart, P J.]] |
[[Category: Ishikawa-Brush, Y.]] | [[Category: Ishikawa-Brush, Y.]] | ||
[[Category: Jiang, J.]] | [[Category: Jiang, J.]] | ||
| - | [[Category: Lafer, E | + | [[Category: Lafer, E M.]] |
[[Category: Prasad, K.]] | [[Category: Prasad, K.]] | ||
[[Category: Sousa, R.]] | [[Category: Sousa, R.]] | ||
| - | [[Category: Taylor, A | + | [[Category: Taylor, A B.]] |
[[Category: alpha helix]] | [[Category: alpha helix]] | ||
[[Category: anti-parallel helix hairpin]] | [[Category: anti-parallel helix hairpin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:38 2008'' |
Revision as of 12:11, 21 February 2008
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Crystal Structure of Auxilin J-Domain
Overview
J-domains are widespread protein interaction modules involved in recruiting and stimulating the activity of Hsp70 family chaperones. We have determined the crystal structure of the J-domain of auxilin, a protein which is involved in uncoating clathrin-coated vesicles. Comparison to the known structures of J-domains from four other proteins reveals that the auxilin J-domain is the most divergent of all J-domain structures described to date. In addition to the canonical J-domain features described previously, the auxilin J-domain contains an extra N-terminal helix and a long loop inserted between helices I and II. The latter loop extends the positively charged surface which forms the Hsc70 binding site, and is shown by directed mutagenesis and surface plasmon resonance to contain side chains important for binding to Hsc70.
About this Structure
1NZ6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Structure-function analysis of the auxilin J-domain reveals an extended Hsc70 interaction interface., Jiang J, Taylor AB, Prasad K, Ishikawa-Brush Y, Hart PJ, Lafer EM, Sousa R, Biochemistry. 2003 May 20;42(19):5748-53. PMID:12741832
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