1nzs
From Proteopedia
(New page: 200px<br /><applet load="1nzs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nzs" /> '''NMR structures of phosphorylated carboxy ter...) |
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'''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state'''<br /> | '''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Visual arrestin binds to the phosphorylated carboxy-terminal region of | + | Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin. |
==About this Structure== | ==About this Structure== | ||
| - | 1NZS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1NZS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZS OCA]. |
==Reference== | ==Reference== | ||
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15111114 15111114] | The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15111114 15111114] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Hargrave, P | + | [[Category: Hargrave, P A.]] |
| - | [[Category: Kisselev, O | + | [[Category: Kisselev, O G.]] |
| - | [[Category: McDowell, J | + | [[Category: McDowell, J H.]] |
[[Category: arrestin]] | [[Category: arrestin]] | ||
[[Category: ball-and-chain]] | [[Category: ball-and-chain]] | ||
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[[Category: signal termination]] | [[Category: signal termination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:11:49 2008'' |
Revision as of 12:11, 21 February 2008
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NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state
Overview
Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin.
About this Structure
1NZS is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:15111114
Page seeded by OCA on Thu Feb 21 14:11:49 2008
