1o7b
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The solution structure of the Link module from human TSG-6, a hyaladherin | + | The solution structure of the Link module from human TSG-6, a hyaladherin with important roles in inflammation and ovulation, has been determined in both its free and hyaluronan-bound conformations. This reveals a well defined hyaluronan-binding groove on one face of the Link module that is closed in the absence of ligand. The groove is lined with amino acids that have been implicated in mediating the interaction with hyaluronan, including two tyrosine residues that appear to form essential intermolecular hydrogen bonds and two basic residues capable of supporting ionic interactions. This is the first structure of a non-enzymic hyaladherin in its active state, and identifies a ligand-induced conformational change that is likely to be conserved across the Link module superfamily. NMR and isothermal titration calorimetry experiments with defined oligosaccharides have allowed us to infer the minimum length of hyaluronan that can be accommodated within the binding site and its polarity in the groove; these data have been used to generate a model of the complex formed between the Link module and a hyaluronan octasaccharide. |
==About this Structure== | ==About this Structure== | ||
| - | 1O7B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure | + | 1O7B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1TSG. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blundell, C | + | [[Category: Blundell, C D.]] |
| - | [[Category: Campbell, I | + | [[Category: Campbell, I D.]] |
| - | [[Category: Day, A | + | [[Category: Day, A J.]] |
| - | [[Category: Kahmann, J | + | [[Category: Kahmann, J D.]] |
| - | [[Category: Pickford, A | + | [[Category: Pickford, A R.]] |
[[Category: Teriete, P.]] | [[Category: Teriete, P.]] | ||
[[Category: carbohydrate-binding domain]] | [[Category: carbohydrate-binding domain]] | ||
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[[Category: link module]] | [[Category: link module]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:14:14 2008'' |
Revision as of 12:14, 21 February 2008
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REFINED SOLUTION STRUCTURE OF THE HUMAN TSG-6 LINK MODULE
Overview
The solution structure of the Link module from human TSG-6, a hyaladherin with important roles in inflammation and ovulation, has been determined in both its free and hyaluronan-bound conformations. This reveals a well defined hyaluronan-binding groove on one face of the Link module that is closed in the absence of ligand. The groove is lined with amino acids that have been implicated in mediating the interaction with hyaluronan, including two tyrosine residues that appear to form essential intermolecular hydrogen bonds and two basic residues capable of supporting ionic interactions. This is the first structure of a non-enzymic hyaladherin in its active state, and identifies a ligand-induced conformational change that is likely to be conserved across the Link module superfamily. NMR and isothermal titration calorimetry experiments with defined oligosaccharides have allowed us to infer the minimum length of hyaluronan that can be accommodated within the binding site and its polarity in the groove; these data have been used to generate a model of the complex formed between the Link module and a hyaluronan octasaccharide.
About this Structure
1O7B is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 1TSG. Full crystallographic information is available from OCA.
Reference
The link module from ovulation- and inflammation-associated protein TSG-6 changes conformation on hyaluronan binding., Blundell CD, Mahoney DJ, Almond A, DeAngelis PL, Kahmann JD, Teriete P, Pickford AR, Campbell ID, Day AJ, J Biol Chem. 2003 Dec 5;278(49):49261-70. Epub 2003 Sep 11. PMID:12972412
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