1oa1
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The hybrid cluster proteins from the sulfate reducing bacteria | + | The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Aragao, D.]] | [[Category: Aragao, D.]] | ||
| - | [[Category: Carrondo, M | + | [[Category: Carrondo, M A.]] |
| - | [[Category: Dongen, W | + | [[Category: Dongen, W M.A M.Van.]] |
[[Category: Frazao, C.]] | [[Category: Frazao, C.]] | ||
| - | [[Category: Hagen, W | + | [[Category: Hagen, W R.]] |
[[Category: Legall, J.]] | [[Category: Legall, J.]] | ||
| - | [[Category: Lindley, P | + | [[Category: Lindley, P F.]] |
| - | [[Category: Liu, M | + | [[Category: Liu, M Y.]] |
[[Category: Macedo, S.]] | [[Category: Macedo, S.]] | ||
| - | [[Category: Mitchell, E | + | [[Category: Mitchell, E P.]] |
| - | [[Category: Romao, C | + | [[Category: Romao, C V.]] |
| - | [[Category: Saraiva, L | + | [[Category: Saraiva, L M.]] |
[[Category: Teixeira, M.]] | [[Category: Teixeira, M.]] | ||
| - | [[Category: Xavier, A | + | [[Category: Xavier, A V.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: SF3]] | [[Category: SF3]] | ||
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[[Category: reduced forms]] | [[Category: reduced forms]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:15:12 2008'' |
Revision as of 12:15, 21 February 2008
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REDUCED HYBRID CLUSTER PROTEIN (HCP) FROM DESULFOVIBRIO VULGARIS HILDENBOROUGH STRUCTURE AT 1.55A RESOLUTION USING SYNCHROTRON RADIATION.
Overview
The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
About this Structure
1OA1 is a Single protein structure of sequence from Desulfovibrio vulgaris with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation., Aragao D, Macedo S, Mitchell EP, Romao CV, Liu MY, Frazao C, Saraiva LM, Xavier AV, LeGall J, van Dongen WM, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2003 May;8(5):540-8. Epub 2003 Feb 15. PMID:12764602
Page seeded by OCA on Thu Feb 21 14:15:12 2008
Categories: Desulfovibrio vulgaris | Single protein | Aragao, D. | Carrondo, M A. | Dongen, W M.A M.Van. | Frazao, C. | Hagen, W R. | Legall, J. | Lindley, P F. | Liu, M Y. | Macedo, S. | Mitchell, E P. | Romao, C V. | Saraiva, L M. | Teixeira, M. | Xavier, A V. | GOL | SF3 | SF4 | Hybrid cluster protein | Reduced forms
