1ocm
From Proteopedia
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==Overview== | ==Overview== | ||
| - | A large group of hydrolytic enzymes, which contain a conserved stretch of | + | A large group of hydrolytic enzymes, which contain a conserved stretch of approximately 130 amino acids designated the amidase signature (AS) sequence, constitutes a super family that is distinct from any other known hydrolase family. AS family enzymes are widespread in nature, ranging from bacteria to humans, and exhibit a variety of biological functions. Here we report the first structure of an AS family enzyme provided by the crystal structure of malonamidase E2 from Bradyrhizobium japonicum. The structure, representing a new protein fold, reveals a previously unidentified Ser-cisSer-Lys catalytic machinery that is absolutely conserved throughout the family. This family of enzymes appears to be evolutionarily distinct but has diverged to acquire a wide spectrum of individual substrate specificities, while maintaining a core structure that supports the catalytic function of the unique triad. Based of the structures of the enzyme in two different inhibited states, an unusual action mechanism of the triad is proposed that accounts for the role of the cis conformation in the triad. |
==About this Structure== | ==About this Structure== | ||
| - | 1OCM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum] with <scene name='pdbligand=POP:'>POP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure | + | 1OCM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_japonicum Bradyrhizobium japonicum] with <scene name='pdbligand=POP:'>POP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. This structure supersedes the now removed PDB entry 1GRK. Known structural/functional Site: <scene name='pdbsite=PP1:Pop+Binding+Site+For+Chain+B'>PP1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OCM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bradyrhizobium japonicum]] | [[Category: Bradyrhizobium japonicum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ha, N | + | [[Category: Ha, N C.]] |
| - | [[Category: Lee, T | + | [[Category: Lee, T H.]] |
| - | [[Category: Oh, B | + | [[Category: Oh, B H.]] |
[[Category: Shin, S.]] | [[Category: Shin, S.]] | ||
[[Category: POP]] | [[Category: POP]] | ||
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[[Category: malonamidase]] | [[Category: malonamidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:16:04 2008'' |
Revision as of 12:16, 21 February 2008
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THE CRYSTAL STRUCTURE OF MALONAMIDASE E2 COVALENTLY COMPLEXED WITH PYROPHOSPHATE FROM BRADYRHIZOBIUM JAPONICUM
Overview
A large group of hydrolytic enzymes, which contain a conserved stretch of approximately 130 amino acids designated the amidase signature (AS) sequence, constitutes a super family that is distinct from any other known hydrolase family. AS family enzymes are widespread in nature, ranging from bacteria to humans, and exhibit a variety of biological functions. Here we report the first structure of an AS family enzyme provided by the crystal structure of malonamidase E2 from Bradyrhizobium japonicum. The structure, representing a new protein fold, reveals a previously unidentified Ser-cisSer-Lys catalytic machinery that is absolutely conserved throughout the family. This family of enzymes appears to be evolutionarily distinct but has diverged to acquire a wide spectrum of individual substrate specificities, while maintaining a core structure that supports the catalytic function of the unique triad. Based of the structures of the enzyme in two different inhibited states, an unusual action mechanism of the triad is proposed that accounts for the role of the cis conformation in the triad.
About this Structure
1OCM is a Single protein structure of sequence from Bradyrhizobium japonicum with as ligand. This structure supersedes the now removed PDB entry 1GRK. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of malonamidase E2 reveals a novel Ser-cisSer-Lys catalytic triad in a new serine hydrolase fold that is prevalent in nature., Shin S, Lee TH, Ha NC, Koo HM, Kim SY, Lee HS, Kim YS, Oh BH, EMBO J. 2002 Jun 3;21(11):2509-16. PMID:12032064
Page seeded by OCA on Thu Feb 21 14:16:04 2008
Categories: Bradyrhizobium japonicum | Single protein | Ha, N C. | Lee, T H. | Oh, B H. | Shin, S. | POP | Amidase | Malonamidase
