1ofx
From Proteopedia
(New page: 200px<br /><applet load="1ofx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ofx, resolution 2.000Å" /> '''CRYSTAL STRUCTURE O...) |
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| - | [[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size=" | + | [[Image:1ofx.jpg|left|200px]]<br /><applet load="1ofx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ofx, resolution 2.000Å" /> | caption="1ofx, resolution 2.000Å" /> | ||
'''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In DNA replication, Okazaki fragments are formed as double-stranded | + | In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation. |
==About this Structure== | ==About this Structure== | ||
| - | 1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SPM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1OFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:22 2008'' |
Revision as of 12:17, 21 February 2008
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CRYSTAL STRUCTURE OF AN OKAZAKI FRAGMENT AT 2 ANGSTROMS RESOLUTION
Overview
In DNA replication, Okazaki fragments are formed as double-stranded intermediates during synthesis of the lagging strand. They are composed of the growing DNA strand primed by RNA and the template strand. The DNA oligonucleotide d(GGGTATACGC) and the chimeric RNA-DNA oligonucleotide r(GCG)d(TATACCC) were combined to form a synthetic Okazaki fragment and its three-dimensional structure was determined by x-ray crystallography. The fragment adopts an overall A-type conformation with 11 residues per turn. Although the base-pair geometry, particularly in the central TATA part, is distorted, there is no evidence for a transition from the A- to the B-type conformation at the junction between RNA.DNA hybrid and DNA duplex. The RNA trimer may, therefore, lock the complete fragment in an A-type conformation.
About this Structure
1OFX is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of an Okazaki fragment at 2-A resolution., Egli M, Usman N, Zhang SG, Rich A, Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):534-8. PMID:1370582
Page seeded by OCA on Thu Feb 21 14:17:22 2008
Categories: Protein complex | Egli, M. | Rich, A. | Usman, N. | Zhang, S. | SPM | A-dna/rna | Double helix
