1omc
From Proteopedia
(New page: 200px<br /><applet load="1omc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1omc" /> '''SOLUTION STRUCTURE OF OMEGA-CONOTOXIN GVIA U...) |
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| - | [[Image:1omc.gif|left|200px]]<br /><applet load="1omc" size=" | + | [[Image:1omc.gif|left|200px]]<br /><applet load="1omc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1omc" /> | caption="1omc" /> | ||
'''SOLUTION STRUCTURE OF OMEGA-CONOTOXIN GVIA USING 2-D NMR SPECTROSCOPY AND RELAXATION MATRIX ANALYSIS'''<br /> | '''SOLUTION STRUCTURE OF OMEGA-CONOTOXIN GVIA USING 2-D NMR SPECTROSCOPY AND RELAXATION MATRIX ANALYSIS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report here the solution structure of omega-conotoxin GVIA, a peptide | + | We report here the solution structure of omega-conotoxin GVIA, a peptide antagonist of the N-type neuronal voltage-sensitive calcium channel. The structure was determined using two-dimensional NMR in combination with distance geometry and restrained molecular dynamics. The full relaxation matrix analysis program MARDIGRAS was used to generate maximum and minimum distance restraints from the crosspeak intensities in NOESY spectra. The 187 restraints obtained were used in conjunction with 23 angle restraints from vicinal coupling constants as input for the structure calculations. The backbones of the best 21 structures match with an average pairwise RMSD of 0.58 A. The structures contain a short segment of triple-stranded beta-sheet involving residues 6-8, 18-21, and 24-27, making this the smallest published peptide structure to contain a triple-stranded beta-sheet. Conotoxins have been shown to be effective neuroprotective agents in animal models of brain ischemia. Our results should aid in the design of novel nonpeptide compounds with potential therapeutic utility. |
==About this Structure== | ==About this Structure== | ||
| - | 1OMC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1OMC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Conus_geographus Conus geographus] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OMC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Conus geographus]] | [[Category: Conus geographus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Basus, V | + | [[Category: Basus, V J.]] |
| - | [[Category: Bradley, E | + | [[Category: Bradley, E K.]] |
| - | [[Category: Davis, J | + | [[Category: Davis, J H.]] |
| - | [[Category: Miljanich, G | + | [[Category: Miljanich, G P.]] |
[[Category: Nadasdi, L.]] | [[Category: Nadasdi, L.]] | ||
[[Category: Ramachandran, J.]] | [[Category: Ramachandran, J.]] | ||
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[[Category: presynaptic neurotoxin]] | [[Category: presynaptic neurotoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:16 2008'' |
Revision as of 12:19, 21 February 2008
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SOLUTION STRUCTURE OF OMEGA-CONOTOXIN GVIA USING 2-D NMR SPECTROSCOPY AND RELAXATION MATRIX ANALYSIS
Overview
We report here the solution structure of omega-conotoxin GVIA, a peptide antagonist of the N-type neuronal voltage-sensitive calcium channel. The structure was determined using two-dimensional NMR in combination with distance geometry and restrained molecular dynamics. The full relaxation matrix analysis program MARDIGRAS was used to generate maximum and minimum distance restraints from the crosspeak intensities in NOESY spectra. The 187 restraints obtained were used in conjunction with 23 angle restraints from vicinal coupling constants as input for the structure calculations. The backbones of the best 21 structures match with an average pairwise RMSD of 0.58 A. The structures contain a short segment of triple-stranded beta-sheet involving residues 6-8, 18-21, and 24-27, making this the smallest published peptide structure to contain a triple-stranded beta-sheet. Conotoxins have been shown to be effective neuroprotective agents in animal models of brain ischemia. Our results should aid in the design of novel nonpeptide compounds with potential therapeutic utility.
About this Structure
1OMC is a Single protein structure of sequence from Conus geographus with as ligand. Full crystallographic information is available from OCA.
Reference
Solution structure of omega-conotoxin GVIA using 2-D NMR spectroscopy and relaxation matrix analysis., Davis JH, Bradley EK, Miljanich GP, Nadasdi L, Ramachandran J, Basus VJ, Biochemistry. 1993 Jul 27;32(29):7396-405. PMID:8338837
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