1aqe
From Proteopedia
| Line 22: | Line 22: | ||
[[Category: point mutant]] | [[Category: point mutant]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:50:47 2007'' |
Revision as of 12:46, 30 October 2007
|
CRYSTAL STRUCTURE OF THE Y73E MUTANT OF CYTOCHROME C OF CLASS III (AMBLER) 26 KD
Overview
A combination of structural, kinetic, and interaction experiments has been, used to study the role of a highly conserved aromatic residue, Tyr73, parallel to the sixth heme axial ligand of heme 4 in multiheme cytochrome, c3 (Mr = 26 000), also called cytochrome cc3 or octaheme cytochrome, from, Desulfovibrio desulfuricans Norway. This residue is expected to be, involved in intermolecular electron transfer and protein-protein, interaction, since heme 4 is described to be the interaction site between, physiological partners. The kinetic experiments show that the Y73E, replacement provokes no significant change in the electron-transfer, reaction with the physiological partner, the [NiFeSe] hydrogenase, but, that the protein-protein interaction between cytochrome c3 (Mr = 26 000), and ... [(full description)]
About this Structure
1AQE is a [Single protein] structure of sequence from [Desulfovibrio desulfuricans] with SO4 and HEM as [ligands]. Structure known Active Sites: HE1, HE2, HE3 and HE4. Full crystallographic information is available from [OCA].
Reference
Structural and kinetic studies of the Y73E mutant of octaheme cytochrome c3 (Mr = 26 000) from Desulfovibrio desulfuricans Norway., Aubert C, Giudici-Orticoni MT, Czjzek M, Haser R, Bruschi M, Dolla A, Biochemistry. 1998 Feb 24;37(8):2120-30. PMID:9485359
Page seeded by OCA on Tue Oct 30 14:50:47 2007
