1ona
From Proteopedia
(New page: 200px<br /><applet load="1ona" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ona, resolution 2.35Å" /> '''CO-CRYSTALS OF CONCA...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ona.jpg|left|200px]]<br /><applet load="1ona" size=" | + | [[Image:1ona.jpg|left|200px]]<br /><applet load="1ona" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ona, resolution 2.35Å" /> | caption="1ona, resolution 2.35Å" /> | ||
'''CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE'''<br /> | '''CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of concanavalin A in complex with the trimannoside | + | The structure of concanavalin A in complex with the trimannoside methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside has been determined in a novel space group. In three of the four subunits of the concanavalin A tetramer, the interactions between the protein and the bound saccharide are essentially identical to those reported previously by other authors (Naismith, J. H., and Field, R. A. (1996) J. Biol. Chem. 271, 972-976). In the fourth subunit, however, the alpha1-->3 linkage has a different conformation, resulting in a different part of the alpha1-->3-linked mannose interacting with essentially the same surface of the protein. Furthermore, significant differences are observed in the quaternary associations of the subunits compared with the saccharide-free structures and other carbohydrate complexes, suggesting that the concanavalin A tetramer is a rather flexible entity. |
==About this Structure== | ==About this Structure== | ||
| - | 1ONA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ONA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONA OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:36 2008'' |
Revision as of 12:19, 21 February 2008
|
CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE
Overview
The structure of concanavalin A in complex with the trimannoside methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside has been determined in a novel space group. In three of the four subunits of the concanavalin A tetramer, the interactions between the protein and the bound saccharide are essentially identical to those reported previously by other authors (Naismith, J. H., and Field, R. A. (1996) J. Biol. Chem. 271, 972-976). In the fourth subunit, however, the alpha1-->3 linkage has a different conformation, resulting in a different part of the alpha1-->3-linked mannose interacting with essentially the same surface of the protein. Furthermore, significant differences are observed in the quaternary associations of the subunits compared with the saccharide-free structures and other carbohydrate complexes, suggesting that the concanavalin A tetramer is a rather flexible entity.
About this Structure
1ONA is a Single protein structure of sequence from Canavalia ensiformis with and as ligands. Full crystallographic information is available from OCA.
Reference
A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes., Loris R, Maes D, Poortmans F, Wyns L, Bouckaert J, J Biol Chem. 1996 Nov 29;271(48):30614-8. PMID:8940035
Page seeded by OCA on Thu Feb 21 14:19:36 2008
Categories: Canavalia ensiformis | Single protein | Bouckaert, J. | Loris, R. | Maes, D. | Poortmans, F. | Wyns, L. | CA | MN | Calcium | Glycoprotein | Manganese | Plant lectin | Signal
