1on6
From Proteopedia
(New page: 200px<br /><applet load="1on6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1on6, resolution 2.30Å" /> '''Crystal structure of...) |
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| - | [[Image:1on6.jpg|left|200px]]<br /><applet load="1on6" size=" | + | [[Image:1on6.jpg|left|200px]]<br /><applet load="1on6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1on6, resolution 2.30Å" /> | caption="1on6, resolution 2.30Å" /> | ||
'''Crystal structure of mouse alpha-1,4-N-acetylhexosaminotransferase (EXTL2) in complex with UDPGlcNAc'''<br /> | '''Crystal structure of mouse alpha-1,4-N-acetylhexosaminotransferase (EXTL2) in complex with UDPGlcNAc'''<br /> | ||
==Overview== | ==Overview== | ||
| - | EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer | + | EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. We have solved the x-ray crystal structure of the catalytic domain of mouse EXTL2 in the apo-form and with donor substrates UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine. In addition, a structure of the ternary complex with UDP and the acceptor substrate analog [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol has been determined. These structures reveal three highly conserved residues, Asn-243, Asp-246, and Arg-293, located at the active site. Mutation of these residues greatly decreases the activity. In the ternary complex, an interaction exists between the beta-phosphate of the UDP leaving group and the acceptor hydroxyl of the substrate that may play a functional role in catalysis. These structures represent the first structures from the exostosin gene family and provide important insight into the mechanisms of alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1ON6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MN, UD1 and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ON6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=UD1:'>UD1</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ON6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dong, J.]] | [[Category: Dong, J.]] | ||
[[Category: Kitagawa, H.]] | [[Category: Kitagawa, H.]] | ||
| - | [[Category: Krahn, J | + | [[Category: Krahn, J M.]] |
[[Category: Negishi, M.]] | [[Category: Negishi, M.]] | ||
| - | [[Category: Pedersen, L | + | [[Category: Pedersen, L C.]] |
| - | [[Category: Pedersen, L | + | [[Category: Pedersen, L G.]] |
[[Category: Sugahara, K.]] | [[Category: Sugahara, K.]] | ||
[[Category: Taniguchi, F.]] | [[Category: Taniguchi, F.]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:35 2008'' |
Revision as of 12:19, 21 February 2008
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Crystal structure of mouse alpha-1,4-N-acetylhexosaminotransferase (EXTL2) in complex with UDPGlcNAc
Overview
EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer reaction of N-acetylglucosamine and N-acetylgalactosamine from the respective UDP-sugars to the non-reducing end of [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol, an acceptor substrate analog of the natural common linker of various glycosylaminoglycans. We have solved the x-ray crystal structure of the catalytic domain of mouse EXTL2 in the apo-form and with donor substrates UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine. In addition, a structure of the ternary complex with UDP and the acceptor substrate analog [glucuronic acid]beta1-3[galactose]beta1-O-naphthalenemethanol has been determined. These structures reveal three highly conserved residues, Asn-243, Asp-246, and Arg-293, located at the active site. Mutation of these residues greatly decreases the activity. In the ternary complex, an interaction exists between the beta-phosphate of the UDP leaving group and the acceptor hydroxyl of the substrate that may play a functional role in catalysis. These structures represent the first structures from the exostosin gene family and provide important insight into the mechanisms of alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis.
About this Structure
1ON6 is a Single protein structure of sequence from Mus musculus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of an alpha 1,4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis., Pedersen LC, Dong J, Taniguchi F, Kitagawa H, Krahn JM, Pedersen LG, Sugahara K, Negishi M, J Biol Chem. 2003 Apr 18;278(16):14420-8. Epub 2003 Jan 31. PMID:12562774
Page seeded by OCA on Thu Feb 21 14:19:35 2008
Categories: Mus musculus | Single protein | Dong, J. | Kitagawa, H. | Krahn, J M. | Negishi, M. | Pedersen, L C. | Pedersen, L G. | Sugahara, K. | Taniguchi, F. | EDO | MN | UD1 | Dxd motif | Rossmann fold
