1aqn

From Proteopedia

Revision as of 12:46, 30 October 2007

SUBTILISIN MUTANT 8324

Overview

Translational initiation factor 3 (IF3) is an RNA helix destabilizing, protein which interacts with strongly conserved sequences in 16S rRNA, one, at the 3' terminus and one in the central domain. It was therefore of, interest to identify particular residues whose exposure changes upon IF3, binding. Chemical and enzymatic probing of central domain nucleotides of, 16S rRNA in 30S ribosomal subunits was carried out in the presence and, absence of IF3. Bases were probed with dimethyl sulfate (DMS), at A(N-1), C(N-3), and G(N-7), and with, N-cyclohexyl-N'-[2-(N-methyl-4-morpholinio)ethyl] carbodiimide, p-toluenesulfonate (CMCT), at G(N-1) and U(N-3). RNase T1 and nuclease S1, were used to probe unpaired nucleotides, and RNase V1 was used to monitor, base-paired or stacked nucleotides. 30S ... [(full description)]

About this Structure

1AQN is a [Single protein] structure of sequence from [Bacillus amyloliquefaciens] with CA, UNX and IPA as [ligands]. Active as [Subtilisin], with EC number [3.4.21.62]. Structure known Active Sites: 169, 206, 217, 218, C22, C87, CA1, CA2 and F50. Full crystallographic information is available from [OCA].

Reference

Escherichia coli initiation factor 3 protein binding to 30S ribosomal subunits alters the accessibility of nucleotides within the conserved central region of 16S rRNA., Muralikrishna P, Wickstrom E, Biochemistry. 1989 Sep 19;28(19):7505-10. PMID:2514787

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