1oo0

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(New page: 200px<br /><applet load="1oo0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oo0, resolution 1.85&Aring;" /> '''Crystal structure of...)
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[[Image:1oo0.gif|left|200px]]<br /><applet load="1oo0" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1oo0.gif|left|200px]]<br /><applet load="1oo0" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1oo0, resolution 1.85&Aring;" />
caption="1oo0, resolution 1.85&Aring;" />
'''Crystal structure of the Drosophila Mago nashi-Y14 complex'''<br />
'''Crystal structure of the Drosophila Mago nashi-Y14 complex'''<br />
==Overview==
==Overview==
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Pre-mRNA splicing is essential for generating mature mRNA and is also, important for subsequent mRNA export and quality control. The splicing, history is imprinted on spliced mRNA through the deposition of a, splicing-dependent multiprotein complex, the exon junction complex (EJC), at approximately 20 nucleotides upstream of exon-exon junctions. The EJC, is a dynamic structure containing proteins functioning in the nuclear, export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and, Y14 are core components of the EJC, and they form a stable heterodimer, that strongly associates with spliced mRNA. Here we report a 1.85, A-resolution structure of the Drosophila Mago-Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA, recognition motif (RRM) is involved in extensive protein-protein, interactions with Mago. This unexpected finding provides important, insights for understanding the molecular mechanisms of EJC assembly and, RRM-mediated protein-protein interactions.
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Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at approximately 20 nucleotides upstream of exon-exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 A-resolution structure of the Drosophila Mago-Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein-protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein-protein interactions.
==About this Structure==
==About this Structure==
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1OO0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with SR, BME, MPD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OO0 OCA].
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1OO0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=SR:'>SR</scene>, <scene name='pdbligand=BME:'>BME</scene>, <scene name='pdbligand=MPD:'>MPD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO0 OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Shi, H.]]
[[Category: Shi, H.]]
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[[Category: Xu, R.M.]]
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[[Category: Xu, R M.]]
[[Category: BME]]
[[Category: BME]]
[[Category: GOL]]
[[Category: GOL]]
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[[Category: splicing]]
[[Category: splicing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:03:19 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:51 2008''

Revision as of 12:19, 21 February 2008

Image:1oo0.gif

1oo0, resolution 1.85Å

Drag the structure with the mouse to rotate

Crystal structure of the Drosophila Mago nashi-Y14 complex

Overview

Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at approximately 20 nucleotides upstream of exon-exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 A-resolution structure of the Drosophila Mago-Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein-protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein-protein interactions.

About this Structure

1OO0 is a Protein complex structure of sequences from Drosophila melanogaster with , , and as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Drosophila Mago nashi-Y14 complex., Shi H, Xu RM, Genes Dev. 2003 Apr 15;17(8):971-6. PMID:12704080

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