1ooi
From Proteopedia
(New page: 200px<br /><applet load="1ooi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ooi, resolution 2.04Å" /> '''Crystal structure of...) |
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| - | [[Image:1ooi.jpg|left|200px]]<br /><applet load="1ooi" size=" | + | [[Image:1ooi.jpg|left|200px]]<br /><applet load="1ooi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ooi, resolution 2.04Å" /> | caption="1ooi, resolution 2.04Å" /> | ||
'''Crystal structure of LUSH from Drosophila melanogaster at pH 6.5'''<br /> | '''Crystal structure of LUSH from Drosophila melanogaster at pH 6.5'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have solved the high-resolution crystal structures of the Drosophila | + | We have solved the high-resolution crystal structures of the Drosophila melanogaster alcohol-binding protein LUSH in complex with a series of short-chain n-alcohols. LUSH is the first known nonenzyme protein with a defined in vivo alcohol-binding function. The structure of LUSH reveals a set of molecular interactions that define a specific alcohol-binding site. A group of amino acids, Thr57, Ser52 and Thr48, form a network of concerted hydrogen bonds between the protein and the alcohol that provides a structural motif to increase alcohol-binding affinity at this site. This motif seems to be conserved in a number of mammalian ligand-gated ion channels that are directly implicated in the pharmacological effects of alcohol. Further, these sequences are found in regions of ion channels that are known to confer alcohol sensitivity. We suggest that the alcohol-binding site in LUSH represents a general model for alcohol-binding sites in proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1OOI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1OOI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OOI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Jones, D | + | [[Category: Jones, D N.M.]] |
| - | [[Category: Kruse, S | + | [[Category: Kruse, S W.]] |
| - | [[Category: Smith, D | + | [[Category: Smith, D P.]] |
[[Category: Zhao, R.]] | [[Category: Zhao, R.]] | ||
[[Category: alcohol]] | [[Category: alcohol]] | ||
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[[Category: odorant-binding protein]] | [[Category: odorant-binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:55 2008'' |
Revision as of 12:19, 21 February 2008
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Crystal structure of LUSH from Drosophila melanogaster at pH 6.5
Overview
We have solved the high-resolution crystal structures of the Drosophila melanogaster alcohol-binding protein LUSH in complex with a series of short-chain n-alcohols. LUSH is the first known nonenzyme protein with a defined in vivo alcohol-binding function. The structure of LUSH reveals a set of molecular interactions that define a specific alcohol-binding site. A group of amino acids, Thr57, Ser52 and Thr48, form a network of concerted hydrogen bonds between the protein and the alcohol that provides a structural motif to increase alcohol-binding affinity at this site. This motif seems to be conserved in a number of mammalian ligand-gated ion channels that are directly implicated in the pharmacological effects of alcohol. Further, these sequences are found in regions of ion channels that are known to confer alcohol sensitivity. We suggest that the alcohol-binding site in LUSH represents a general model for alcohol-binding sites in proteins.
About this Structure
1OOI is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of a specific alcohol-binding site defined by the odorant binding protein LUSH from Drosophila melanogaster., Kruse SW, Zhao R, Smith DP, Jones DN, Nat Struct Biol. 2003 Sep;10(9):694-700. Epub 2003 Jul 27. PMID:12881720
Page seeded by OCA on Thu Feb 21 14:19:55 2008
