1opo
From Proteopedia
(New page: 200px<br /><applet load="1opo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1opo, resolution 3.20Å" /> '''THE STRUCTURE OF CAR...) |
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| - | [[Image:1opo.gif|left|200px]]<br /><applet load="1opo" size=" | + | [[Image:1opo.gif|left|200px]]<br /><applet load="1opo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1opo, resolution 3.20Å" /> | caption="1opo, resolution 3.20Å" /> | ||
'''THE STRUCTURE OF CARNATION MOTTLE VIRUS'''<br /> | '''THE STRUCTURE OF CARNATION MOTTLE VIRUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the Carnation Mottle Virus (CMtV) capsid protein has been | + | The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway. |
==About this Structure== | ==About this Structure== | ||
| - | 1OPO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carnation_mottle_virus Carnation mottle virus] with SO4 and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1OPO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Carnation_mottle_virus Carnation mottle virus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPO OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
[[Category: Fry, E.]] | [[Category: Fry, E.]] | ||
| - | [[Category: Mikhailov, A | + | [[Category: Mikhailov, A M.]] |
[[Category: Morgunova, E.]] | [[Category: Morgunova, E.]] | ||
[[Category: Stuart, D.]] | [[Category: Stuart, D.]] | ||
| - | [[Category: Vainshtein, B | + | [[Category: Vainshtein, B K.]] |
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
| - | [[Category: mashchuk, V | + | [[Category: mashchuk, V Stel.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: x-ray diffraction]] | [[Category: x-ray diffraction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:16 2008'' |
Revision as of 12:20, 21 February 2008
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THE STRUCTURE OF CARNATION MOTTLE VIRUS
Overview
The structure of the Carnation Mottle Virus (CMtV) capsid protein has been determined at 3.2 A resolution by the method of molecular replacement. Three-dimensional data were collected from a small number of crystals (sp.g. I23, a = 382.6 A) using the synchrotron radiation with an image plate as detector. The coordinates of Tomato Bushy Stunt Virus (TBSV) were used as a searching model. Refinement of the coordinates of 7,479 non-hydrogen atoms performed by the program XPLOR, has led to an R-factor of 18.3%. It was found that the amino acid chain fold of capsid protein is very similar to that in other icosahedral viruses. However, there are some differences in the contact regions between protein subunits and also the lack of the beta-annulus around the 3-fold icosahedral axes. The structural and biochemical results lead us to consider an alternative assembly pathway.
About this Structure
1OPO is a Single protein structure of sequence from Carnation mottle virus with and as ligands. Full crystallographic information is available from OCA.
Reference
The atomic structure of Carnation Mottle Virus capsid protein., Morgunova EYu, Dauter Z, Fry E, Stuart DI, Stel'mashchuk VYa, Mikhailov AM, Wilson KS, Vainshtein BK, FEBS Lett. 1994 Feb 7;338(3):267-71. PMID:8307192
Page seeded by OCA on Thu Feb 21 14:20:16 2008
Categories: Carnation mottle virus | Single protein | Dauter, Z. | Fry, E. | Mikhailov, A M. | Morgunova, E. | Stuart, D. | Vainshtein, B K. | Wilson, K S. | Mashchuk, V Stel. | CA | SO4 | Carmovirus | Icosahedral virus | Plant virus | Tomato bushy stunt virus | Virus/viral protein | X-ray diffraction
