1orc
From Proteopedia
(New page: 200px<br /><applet load="1orc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1orc, resolution 1.54Å" /> '''CRO REPRESSOR INSERT...) |
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| - | [[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size=" | + | [[Image:1orc.gif|left|200px]]<br /><applet load="1orc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1orc, resolution 1.54Å" /> | caption="1orc, resolution 1.54Å" /> | ||
'''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''<br /> | '''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A rationally designed, genetically engineered, monomeric form of the Cro | + | A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function. |
==About this Structure== | ==About this Structure== | ||
| - | 1ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http:// | + | 1ORC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Albright, R | + | [[Category: Albright, R A.]] |
| - | [[Category: Matthews, B | + | [[Category: Matthews, B W.]] |
| - | [[Category: Mossing, M | + | [[Category: Mossing, M C.]] |
[[Category: gene regulating protein]] | [[Category: gene regulating protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:43 2008'' |
Revision as of 12:20, 21 February 2008
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CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]
Overview
A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function.
About this Structure
1ORC is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer., Albright RA, Mossing MC, Matthews BW, Biochemistry. 1996 Jan 23;35(3):735-42. PMID:8547253
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