1orl
From Proteopedia
(New page: 200px<br /><applet load="1orl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1orl" /> '''1H NMR structure determination of Viscotoxin...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1orl.jpg|left|200px]]<br /><applet load="1orl" size=" | + | [[Image:1orl.jpg|left|200px]]<br /><applet load="1orl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1orl" /> | caption="1orl" /> | ||
'''1H NMR structure determination of Viscotoxin C1'''<br /> | '''1H NMR structure determination of Viscotoxin C1'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The high resolution three-dimensional structure of the newly discovered | + | The high resolution three-dimensional structure of the newly discovered plant viscotoxin C1, from the Asiatic Viscum album ssp. Coloratum ohwi, has been determined in solution by (1)H NMR spectroscopy at pH 3.6 and 285 K. The viscotoxin C1-fold, consisting of a helix-turn-helix motif and a short stretch of an antiparralel beta-sheet is very similar to that found for the highly similar viscotoxins A2 and A3 and for other related thionins. Different functional properties of members of the thionin family are discussed here in light of the structural and electrostatic properties. Among the very homologous family of alpha- and beta-thionins, known for their antimicrobial activity, the viscotoxin subfamily differs from the other members because of its high toxicity against tumoral cells. Key residues for the modulation of viscotoxin cytotoxicity have been identified on the basis of sequence and structural alignment. |
==About this Structure== | ==About this Structure== | ||
| - | 1ORL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http:// | + | 1ORL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORL OCA]. |
==Reference== | ==Reference== | ||
| Line 24: | Line 24: | ||
[[Category: helix-turn-helix]] | [[Category: helix-turn-helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:49 2008'' |
Revision as of 12:20, 21 February 2008
|
1H NMR structure determination of Viscotoxin C1
Overview
The high resolution three-dimensional structure of the newly discovered plant viscotoxin C1, from the Asiatic Viscum album ssp. Coloratum ohwi, has been determined in solution by (1)H NMR spectroscopy at pH 3.6 and 285 K. The viscotoxin C1-fold, consisting of a helix-turn-helix motif and a short stretch of an antiparralel beta-sheet is very similar to that found for the highly similar viscotoxins A2 and A3 and for other related thionins. Different functional properties of members of the thionin family are discussed here in light of the structural and electrostatic properties. Among the very homologous family of alpha- and beta-thionins, known for their antimicrobial activity, the viscotoxin subfamily differs from the other members because of its high toxicity against tumoral cells. Key residues for the modulation of viscotoxin cytotoxicity have been identified on the basis of sequence and structural alignment.
About this Structure
1ORL is a Single protein structure of sequence from Viscum album. Full crystallographic information is available from OCA.
Reference
NMR solution structure of viscotoxin C1 from Viscum album species Coloratum ohwi: toward a structure-function analysis of viscotoxins., Romagnoli S, Fogolari F, Catalano M, Zetta L, Schaller G, Urech K, Giannattasio M, Ragona L, Molinari H, Biochemistry. 2003 Nov 4;42(43):12503-10. PMID:14580196
Page seeded by OCA on Thu Feb 21 14:20:49 2008
