1osn
From Proteopedia
(New page: 200px<br /><applet load="1osn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1osn, resolution 3.20Å" /> '''Crystal structure of...) |
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| - | [[Image:1osn.jpg|left|200px]]<br /><applet load="1osn" size=" | + | [[Image:1osn.jpg|left|200px]]<br /><applet load="1osn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1osn, resolution 3.20Å" /> | caption="1osn, resolution 3.20Å" /> | ||
'''Crystal structure of Varicella zoster virus thymidine kinase in complex with BVDU-MP and ADP'''<br /> | '''Crystal structure of Varicella zoster virus thymidine kinase in complex with BVDU-MP and ADP'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Herpes virus thymidine kinases are responsible for the activation of | + | Herpes virus thymidine kinases are responsible for the activation of nucleoside antiviral drugs including (E)-5-(2-bromovinyl)-2'-deoxyuridine. Such viral thymidine kinases (tk), beside having a broader substrate specificity compared with host cell enzymes, also show significant variation in nucleoside phosphorylation among themselves. We have determined the crystal structure of Varicella zoster virus (VZV, human herpes virus 3) thymidine kinase complexed with (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate and ADP. Differences in the conformation of a loop region (residues 55-61) and the position of two alpha-helices at the subunit interface of VZV-tk compared with the herpes simplex virus type 1 (human herpes virus 1) enzyme give rise to changes in the positioning of residues such as tyrosine 66 and glutamine 90, which hydrogen bond to the substrate in the active site. Such changes in combination with the substitution in VZV-tk of two phenylalanine residues (in place of a tyrosine and methionine), which sandwich the substrate pyrimidine ring, cause an alteration in the positioning of the base. The interaction of the (E)-5-(2-bromovinyl)-2'-deoxyuridine deoxyribose ring with the protein is altered by substitution of tyrosine 21 and phenylalanine 139 (analagous to herpes simplex virus type 1 histidine 58 and tyrosine 172), which may explain some of the differences in nucleoside sugar selectivity between both enzymes. The altered active site architecture may also account for the differences in the substrate activity of ganciclovir for the two thymidine kinases. These data should be of use in the design of novel antiherpes and antitumor drugs. |
==About this Structure== | ==About this Structure== | ||
| - | 1OSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_3 Human herpesvirus 3] with ADP and BVP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] Full crystallographic information is available from [http:// | + | 1OSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_3 Human herpesvirus 3] with <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=BVP:'>BVP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Thymidine kinase]] | [[Category: Thymidine kinase]] | ||
[[Category: Balzarini, J.]] | [[Category: Balzarini, J.]] | ||
| - | [[Category: Bird, L | + | [[Category: Bird, L E.]] |
[[Category: Degreve, B.]] | [[Category: Degreve, B.]] | ||
| - | [[Category: Leslie, K | + | [[Category: Leslie, K D.]] |
[[Category: Ren, J.]] | [[Category: Ren, J.]] | ||
| - | [[Category: Stammers, D | + | [[Category: Stammers, D K.]] |
[[Category: Wright, A.]] | [[Category: Wright, A.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: thymidine kinase]] | [[Category: thymidine kinase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:08 2008'' |
Revision as of 12:21, 21 February 2008
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Crystal structure of Varicella zoster virus thymidine kinase in complex with BVDU-MP and ADP
Overview
Herpes virus thymidine kinases are responsible for the activation of nucleoside antiviral drugs including (E)-5-(2-bromovinyl)-2'-deoxyuridine. Such viral thymidine kinases (tk), beside having a broader substrate specificity compared with host cell enzymes, also show significant variation in nucleoside phosphorylation among themselves. We have determined the crystal structure of Varicella zoster virus (VZV, human herpes virus 3) thymidine kinase complexed with (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-monophosphate and ADP. Differences in the conformation of a loop region (residues 55-61) and the position of two alpha-helices at the subunit interface of VZV-tk compared with the herpes simplex virus type 1 (human herpes virus 1) enzyme give rise to changes in the positioning of residues such as tyrosine 66 and glutamine 90, which hydrogen bond to the substrate in the active site. Such changes in combination with the substitution in VZV-tk of two phenylalanine residues (in place of a tyrosine and methionine), which sandwich the substrate pyrimidine ring, cause an alteration in the positioning of the base. The interaction of the (E)-5-(2-bromovinyl)-2'-deoxyuridine deoxyribose ring with the protein is altered by substitution of tyrosine 21 and phenylalanine 139 (analagous to herpes simplex virus type 1 histidine 58 and tyrosine 172), which may explain some of the differences in nucleoside sugar selectivity between both enzymes. The altered active site architecture may also account for the differences in the substrate activity of ganciclovir for the two thymidine kinases. These data should be of use in the design of novel antiherpes and antitumor drugs.
About this Structure
1OSN is a Single protein structure of sequence from Human herpesvirus 3 with and as ligands. Active as Thymidine kinase, with EC number 2.7.1.21 Full crystallographic information is available from OCA.
Reference
Crystal structure of varicella zoster virus thymidine kinase., Bird LE, Ren J, Wright A, Leslie KD, Degreve B, Balzarini J, Stammers DK, J Biol Chem. 2003 Jul 4;278(27):24680-7. Epub 2003 Apr 9. PMID:12686543
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