1ouw
From Proteopedia
(New page: 200px<br /><applet load="1ouw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ouw, resolution 1.37Å" /> '''Crystal structure of...) |
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| - | [[Image:1ouw.gif|left|200px]]<br /><applet load="1ouw" size=" | + | [[Image:1ouw.gif|left|200px]]<br /><applet load="1ouw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ouw, resolution 1.37Å" /> | caption="1ouw, resolution 1.37Å" /> | ||
'''Crystal structure of Calystegia sepium agglutinin'''<br /> | '''Crystal structure of Calystegia sepium agglutinin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The high number of quaternary structures observed for lectins highlights | + | The high number of quaternary structures observed for lectins highlights the important role of these oligomeric assemblies during carbohydrate recognition events. Although a large diversity in the mode of association of lectin subunits is frequently observed, the oligomeric assemblies of plant lectins display small variations within a single family. The crystal structure of the mannose-binding jacalin-related lectin from Calystegia sepium (Calsepa) has been determined at 1.37-A resolution. Calsepa exhibits the same beta-prism fold as identified previously for other members of the family, but the shape and the hydrophobic character of its carbohydrate-binding site is unlike that of other members, consistent with surface plasmon resonance analysis showing a preference for methylated sugars. Calsepa reveals a novel dimeric assembly markedly dissimilar to those described earlier for Heltuba and jacalin but mimics the canonical 12-stranded beta-sandwich dimer found in legume lectins. The present structure exemplifies the adaptability of the beta-prism building block in the evolution of plant lectins and highlights the biological role of these quaternary structures for carbohydrate recognition. |
==About this Structure== | ==About this Structure== | ||
| - | 1OUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium] with MLT, EDO and IMD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1OUW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Calystegia_sepium Calystegia sepium] with <scene name='pdbligand=MLT:'>MLT</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=IMD:'>IMD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OUW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Calystegia sepium]] | [[Category: Calystegia sepium]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Astoul, C | + | [[Category: Astoul, C H.]] |
[[Category: Barre, A.]] | [[Category: Barre, A.]] | ||
[[Category: Bourne, Y.]] | [[Category: Bourne, Y.]] | ||
| - | [[Category: Damme, E | + | [[Category: Damme, E J.M van.]] |
| - | [[Category: Peumans, W | + | [[Category: Peumans, W J.]] |
[[Category: Roig-Zamboni, V.]] | [[Category: Roig-Zamboni, V.]] | ||
[[Category: Rouge, P.]] | [[Category: Rouge, P.]] | ||
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[[Category: mannose-binding]] | [[Category: mannose-binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:21:55 2008'' |
Revision as of 12:21, 21 February 2008
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Crystal structure of Calystegia sepium agglutinin
Overview
The high number of quaternary structures observed for lectins highlights the important role of these oligomeric assemblies during carbohydrate recognition events. Although a large diversity in the mode of association of lectin subunits is frequently observed, the oligomeric assemblies of plant lectins display small variations within a single family. The crystal structure of the mannose-binding jacalin-related lectin from Calystegia sepium (Calsepa) has been determined at 1.37-A resolution. Calsepa exhibits the same beta-prism fold as identified previously for other members of the family, but the shape and the hydrophobic character of its carbohydrate-binding site is unlike that of other members, consistent with surface plasmon resonance analysis showing a preference for methylated sugars. Calsepa reveals a novel dimeric assembly markedly dissimilar to those described earlier for Heltuba and jacalin but mimics the canonical 12-stranded beta-sandwich dimer found in legume lectins. The present structure exemplifies the adaptability of the beta-prism building block in the evolution of plant lectins and highlights the biological role of these quaternary structures for carbohydrate recognition.
About this Structure
1OUW is a Single protein structure of sequence from Calystegia sepium with , and as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of the Calystegia sepium agglutinin reveals a novel quaternary arrangement of lectin subunits with a beta-prism fold., Bourne Y, Roig-Zamboni V, Barre A, Peumans WJ, Astoul CH, Van Damme EJ, Rouge P, J Biol Chem. 2004 Jan 2;279(1):527-33. Epub 2003 Oct 15. PMID:14561768
Page seeded by OCA on Thu Feb 21 14:21:55 2008
Categories: Calystegia sepium | Single protein | Astoul, C H. | Barre, A. | Bourne, Y. | Damme, E J.M van. | Peumans, W J. | Roig-Zamboni, V. | Rouge, P. | EDO | IMD | MLT | Agglutinin | Beta-prism fold | Jacalin-related | Lectin | Mannose-binding
