1ox8
From Proteopedia
(New page: 200px<br /><applet load="1ox8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ox8, resolution 2.2Å" /> '''Crystal structure of ...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1ox8.jpg|left|200px]]<br /><applet load="1ox8" size=" | + | [[Image:1ox8.jpg|left|200px]]<br /><applet load="1ox8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ox8, resolution 2.2Å" /> | caption="1ox8, resolution 2.2Å" /> | ||
'''Crystal structure of SspB'''<br /> | '''Crystal structure of SspB'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In prokaryotes, incomplete or misfolded polypeptides emanating from a | + | In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates. |
==About this Structure== | ==About this Structure== | ||
| - | 1OX8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o127:h6 Escherichia coli o127:h6]. Full crystallographic information is available from [http:// | + | 1OX8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_o127:h6 Escherichia coli o127:h6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OX8 OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Escherichia coli o127:h6]] | [[Category: Escherichia coli o127:h6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Eck, M | + | [[Category: Eck, M J.]] |
| - | [[Category: Song, H | + | [[Category: Song, H K.]] |
[[Category: rna-binding property]] | [[Category: rna-binding property]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:41 2008'' |
Revision as of 12:22, 21 February 2008
|
Crystal structure of SspB
Overview
In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates.
About this Structure
1OX8 is a Single protein structure of sequence from Escherichia coli o127:h6. Full crystallographic information is available from OCA.
Reference
Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine., Song HK, Eck MJ, Mol Cell. 2003 Jul;12(1):75-86. PMID:12887894
Page seeded by OCA on Thu Feb 21 14:22:41 2008
