1oxe
From Proteopedia
(New page: 200px<br /><applet load="1oxe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oxe, resolution 1.15Å" /> '''Expansion of the Gen...) |
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| - | [[Image:1oxe.jpg|left|200px]]<br /><applet load="1oxe" size=" | + | [[Image:1oxe.jpg|left|200px]]<br /><applet load="1oxe" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1oxe, resolution 1.15Å" /> | caption="1oxe, resolution 1.15Å" /> | ||
'''Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins'''<br /> | '''Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Much effort has been dedicated to the design of significantly red shifted | + | Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1OXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1012 Cfp marker plasmid pwm1012]. Full crystallographic information is available from [http:// | + | 1OXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cfp_marker_plasmid_pwm1012 Cfp marker plasmid pwm1012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OXE OCA]. |
==Reference== | ==Reference== | ||
| - | Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., | + | Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12729742 12729742] |
[[Category: Cfp marker plasmid pwm1012]] | [[Category: Cfp marker plasmid pwm1012]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Azim, M | + | [[Category: Azim, M K.]] |
| - | [[Category: Bae, J | + | [[Category: Bae, J Hyun.]] |
[[Category: Budisa, N.]] | [[Category: Budisa, N.]] | ||
| - | [[Category: Holak, T | + | [[Category: Holak, T A.]] |
[[Category: Huber, R.]] | [[Category: Huber, R.]] | ||
[[Category: Jung, G.]] | [[Category: Jung, G.]] | ||
| - | [[Category: Kim, J | + | [[Category: Kim, J S.]] |
[[Category: Moroder, L.]] | [[Category: Moroder, L.]] | ||
[[Category: Rubini, M.]] | [[Category: Rubini, M.]] | ||
| - | [[Category: Seifert, M | + | [[Category: Seifert, M H.]] |
[[Category: Wiegand, G.]] | [[Category: Wiegand, G.]] | ||
[[Category: Zumbusch, A.]] | [[Category: Zumbusch, A.]] | ||
| Line 31: | Line 31: | ||
[[Category: tryptophan]] | [[Category: tryptophan]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:43 2008'' |
Revision as of 12:22, 21 February 2008
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Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins
Overview
Much effort has been dedicated to the design of significantly red shifted variants of the green fluorescent protein (GFP) from Aequoria victora (av). These approaches have been based on classical engineering with the 20 canonical amino acids. We report here an expansion of these efforts by incorporation of an amino substituted variant of tryptophan into the "cyan" GFP mutant, which turned it into a "gold" variant. This variant possesses a red shift in emission unprecedented for any avFP, similar to "red" FPs, but with enhanced stability and a very low aggregation tendency. An increasing number of non-natural amino acids are available for chromophore redesign (by engineering of the genetic code) and enable new general strategies to generate novel classes of tailor-made GFP proteins.
About this Structure
1OXE is a Single protein structure of sequence from Cfp marker plasmid pwm1012. Full crystallographic information is available from OCA.
Reference
Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins., Bae JH, Rubini M, Jung G, Wiegand G, Seifert MH, Azim MK, Kim JS, Zumbusch A, Holak TA, Moroder L, Huber R, Budisa N, J Mol Biol. 2003 May 16;328(5):1071-81. PMID:12729742
Page seeded by OCA on Thu Feb 21 14:22:43 2008
Categories: Cfp marker plasmid pwm1012 | Single protein | Azim, M K. | Bae, J Hyun. | Budisa, N. | Holak, T A. | Huber, R. | Jung, G. | Kim, J S. | Moroder, L. | Rubini, M. | Seifert, M H. | Wiegand, G. | Zumbusch, A. | Amino acid incorporation | Chromophore | Genetic code | Green fluorescent protein | Tryptophan
