1oyg

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(New page: 200px<br /><applet load="1oyg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1oyg, resolution 1.50&Aring;" /> '''Crystal structure of...)
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caption="1oyg, resolution 1.50&Aring;" />
caption="1oyg, resolution 1.50&Aring;" />
'''Crystal structure of Bacillus subtilis levansucrase'''<br />
'''Crystal structure of Bacillus subtilis levansucrase'''<br />
==Overview==
==Overview==
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Many bacteria and about 40,000 plant species form primary carbohydrate, reserves based on fructan; these polymers of beta-D-fructofuranose are, thought to confer tolerance to drought and frost in plants. Microbial, fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose, by levansucrase, which is able to catalyze both sucrose hydrolysis and, levan polymerization. The crystal structure of Bacillus subtilis, levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold, beta-propeller topology with a deep, negatively charged central pocket., Arg360, a residue essential for polymerase activity, lies in a, solvent-exposed site adjacent to the central pocket. Mutagenesis data and, the sucrose-bound structure of inactive levansucrase E342A, at a, resolution of 2.1 A, strongly suggest that three conserved acidic side, chains in the central pocket are critical for catalysis, and presumably, function as nucleophile (Asp86) and general acid (Glu342), or stabilize, the transition state (Asp247).
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Many bacteria and about 40,000 plant species form primary carbohydrate reserves based on fructan; these polymers of beta-D-fructofuranose are thought to confer tolerance to drought and frost in plants. Microbial fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose by levansucrase, which is able to catalyze both sucrose hydrolysis and levan polymerization. The crystal structure of Bacillus subtilis levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold beta-propeller topology with a deep, negatively charged central pocket. Arg360, a residue essential for polymerase activity, lies in a solvent-exposed site adjacent to the central pocket. Mutagenesis data and the sucrose-bound structure of inactive levansucrase E342A, at a resolution of 2.1 A, strongly suggest that three conserved acidic side chains in the central pocket are critical for catalysis, and presumably function as nucleophile (Asp86) and general acid (Glu342), or stabilize the transition state (Asp247).
==About this Structure==
==About this Structure==
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1OYG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with CA and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OYG OCA].
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1OYG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OYG OCA].
==Reference==
==Reference==
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[[Category: levansucrase]]
[[Category: levansucrase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:17:59 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:11 2008''

Revision as of 12:23, 21 February 2008

Image:1oyg.jpg

1oyg, resolution 1.50Å

Drag the structure with the mouse to rotate

Crystal structure of Bacillus subtilis levansucrase

Overview

Many bacteria and about 40,000 plant species form primary carbohydrate reserves based on fructan; these polymers of beta-D-fructofuranose are thought to confer tolerance to drought and frost in plants. Microbial fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose by levansucrase, which is able to catalyze both sucrose hydrolysis and levan polymerization. The crystal structure of Bacillus subtilis levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold beta-propeller topology with a deep, negatively charged central pocket. Arg360, a residue essential for polymerase activity, lies in a solvent-exposed site adjacent to the central pocket. Mutagenesis data and the sucrose-bound structure of inactive levansucrase E342A, at a resolution of 2.1 A, strongly suggest that three conserved acidic side chains in the central pocket are critical for catalysis, and presumably function as nucleophile (Asp86) and general acid (Glu342), or stabilize the transition state (Asp247).

About this Structure

1OYG is a Single protein structure of sequence from Bacillus subtilis with and as ligands. Active as Levansucrase, with EC number 2.4.1.10 Full crystallographic information is available from OCA.

Reference

Structural framework of fructosyl transfer in Bacillus subtilis levansucrase., Meng G, Futterer K, Nat Struct Biol. 2003 Nov;10(11):935-41. Epub 2003 Sep 28. PMID:14517548

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