1p16
From Proteopedia
(New page: 200px<br /><applet load="1p16" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p16, resolution 2.70Å" /> '''Structure of an mRNA...) |
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| - | [[Image:1p16.gif|left|200px]]<br /><applet load="1p16" size=" | + | [[Image:1p16.gif|left|200px]]<br /><applet load="1p16" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p16, resolution 2.70Å" /> | caption="1p16, resolution 2.70Å" /> | ||
'''Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II'''<br /> | '''Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 | + | The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 cocrystallized with a carboxy-terminal domain (CTD) peptide composed of four Ser5-PO4 YSPTSPS heptad repeats illuminates distinct CTD-docking sites localized to the Cgt1 N-terminal nucleotidyl transferase domain. Tyr1, Pro3, Pro6, and Ser5-PO4 side chains from each of two YSPTSPS repeats contribute to the interface. Comparison to the Pin1-CTD structure shows that the CTD can assume markedly different conformations that are templated by particular binding partners. Structural plasticity combined with remodeling of CTD primary structure by kinases and phosphatases provides a versatile mechanism by which the CTD can recruit structurally dissimilar proteins during transcription. A binding site for the RNA triphosphatase component of the capping apparatus was also uncovered within the Cgt1 OB domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1P16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with PO4, G and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] Full crystallographic information is available from [http:// | + | 1P16 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans] with <scene name='pdbligand=PO4:'>PO4</scene>, <scene name='pdbligand=G:'>G</scene> and <scene name='pdbligand=GTP:'>GTP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_guanylyltransferase mRNA guanylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.50 2.7.7.50] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P16 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: mRNA guanylyltransferase]] | [[Category: mRNA guanylyltransferase]] | ||
[[Category: Fabrega, C.]] | [[Category: Fabrega, C.]] | ||
| - | [[Category: Lima, C | + | [[Category: Lima, C D.]] |
[[Category: Shen, V.]] | [[Category: Shen, V.]] | ||
[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:58 2008'' |
Revision as of 12:23, 21 February 2008
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Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II
Overview
The 2.7 A structure of Candida albicans RNA guanylyltransferase Cgt1 cocrystallized with a carboxy-terminal domain (CTD) peptide composed of four Ser5-PO4 YSPTSPS heptad repeats illuminates distinct CTD-docking sites localized to the Cgt1 N-terminal nucleotidyl transferase domain. Tyr1, Pro3, Pro6, and Ser5-PO4 side chains from each of two YSPTSPS repeats contribute to the interface. Comparison to the Pin1-CTD structure shows that the CTD can assume markedly different conformations that are templated by particular binding partners. Structural plasticity combined with remodeling of CTD primary structure by kinases and phosphatases provides a versatile mechanism by which the CTD can recruit structurally dissimilar proteins during transcription. A binding site for the RNA triphosphatase component of the capping apparatus was also uncovered within the Cgt1 OB domain.
About this Structure
1P16 is a Single protein structure of sequence from Candida albicans with , and as ligands. Active as mRNA guanylyltransferase, with EC number 2.7.7.50 Full crystallographic information is available from OCA.
Reference
Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II., Fabrega C, Shen V, Shuman S, Lima CD, Mol Cell. 2003 Jun;11(6):1549-61. PMID:12820968
Page seeded by OCA on Thu Feb 21 14:23:58 2008
Categories: Candida albicans | Single protein | MRNA guanylyltransferase | Fabrega, C. | Lima, C D. | Shen, V. | Shuman, S. | G | GTP | PO4 | Capping | Ctd | Guanylyltransferase | Mrna | Rna polymerase ii | Transcription
