1p24
From Proteopedia
(New page: 200px<br /><applet load="1p24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p24, resolution 3.02Å" /> '''Crystal structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1p24.gif|left|200px]]<br /><applet load="1p24" size=" | + | [[Image:1p24.gif|left|200px]]<br /><applet load="1p24" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p24, resolution 3.02Å" /> | caption="1p24, resolution 3.02Å" /> | ||
'''Crystal structure of cobalt(II)-d(GGCGCC)2'''<br /> | '''Crystal structure of cobalt(II)-d(GGCGCC)2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Three novel X-ray crystal structures for the DNA hexamer d(GGCGCC) in the | + | Three novel X-ray crystal structures for the DNA hexamer d(GGCGCC) in the B-form complexed to divalent cobalt, nickel and zinc ions have been determined to a resolution of 2.9-3.0 A. The structures were isomorphous and had five DNA strands and five metal cations per asymmetric unit. In all three cases, divalent metal cations were coordinated only to the terminal guanine residue at the N(7) position, with no metal ions binding to non-terminal guanine positions. Water molecules bound to the metal cations interacted with neighboring guanine residues 3' to the ones to which the cations were coordinated, affecting the propeller twist. Even though DNA occupied only about 35% of the unit cell volume, it is interesting that the few interactions involving the metal cations were sufficient to stabilize the crystal lattice. As well as lending support to the proposal that these metals do not coordinate to B-DNA in a stable manner, the results presented here also extend the crystallographic evidence for this phenomenon to the GGC and CGC sequences for all three metal cations. |
==About this Structure== | ==About this Structure== | ||
| - | 1P24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CO as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1P24 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CO:'>CO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P24 OCA]. |
==Reference== | ==Reference== | ||
Cobalt(II), nickel(II) and zinc(II) do not bind to intra-helical N(7) guanine positions in the B-form crystal structure of d(GGCGCC)., Labiuk SL, Delbaere LT, Lee JS, J Biol Inorg Chem. 2003 Sep;8(7):715-20. Epub 2003 Jul 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14505075 14505075] | Cobalt(II), nickel(II) and zinc(II) do not bind to intra-helical N(7) guanine positions in the B-form crystal structure of d(GGCGCC)., Labiuk SL, Delbaere LT, Lee JS, J Biol Inorg Chem. 2003 Sep;8(7):715-20. Epub 2003 Jul 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14505075 14505075] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Delbaere, L | + | [[Category: Delbaere, L T.]] |
| - | [[Category: Labiuk, S | + | [[Category: Labiuk, S L.]] |
| - | [[Category: Lee, J | + | [[Category: Lee, J S.]] |
[[Category: CO]] | [[Category: CO]] | ||
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: cobalt binding]] | [[Category: cobalt binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:24:16 2008'' |
Revision as of 12:24, 21 February 2008
|
Crystal structure of cobalt(II)-d(GGCGCC)2
Overview
Three novel X-ray crystal structures for the DNA hexamer d(GGCGCC) in the B-form complexed to divalent cobalt, nickel and zinc ions have been determined to a resolution of 2.9-3.0 A. The structures were isomorphous and had five DNA strands and five metal cations per asymmetric unit. In all three cases, divalent metal cations were coordinated only to the terminal guanine residue at the N(7) position, with no metal ions binding to non-terminal guanine positions. Water molecules bound to the metal cations interacted with neighboring guanine residues 3' to the ones to which the cations were coordinated, affecting the propeller twist. Even though DNA occupied only about 35% of the unit cell volume, it is interesting that the few interactions involving the metal cations were sufficient to stabilize the crystal lattice. As well as lending support to the proposal that these metals do not coordinate to B-DNA in a stable manner, the results presented here also extend the crystallographic evidence for this phenomenon to the GGC and CGC sequences for all three metal cations.
About this Structure
1P24 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Cobalt(II), nickel(II) and zinc(II) do not bind to intra-helical N(7) guanine positions in the B-form crystal structure of d(GGCGCC)., Labiuk SL, Delbaere LT, Lee JS, J Biol Inorg Chem. 2003 Sep;8(7):715-20. Epub 2003 Jul 9. PMID:14505075
Page seeded by OCA on Thu Feb 21 14:24:16 2008
