1p4m
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Riboflavin kinase (RFK) is an essential enzyme catalyzing the | + | Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B(2)) to form FMN, an obligatory step in vitamin B(2) utilization and flavin cofactor synthesis. The structure of human RFK revealed a six-stranded antiparallel beta barrel core structurally similar to the riboflavin synthase/ferredoxin reductase FAD binding domain fold. The binding site of an intrinsically bound MgADP defines a novel nucleotide binding motif that encompasses a loop, a 3(10) helix, and a reverse turn followed by a short beta strand. This active site loop forms an arch with ATP and riboflavin binding at the opposite side and the phosphoryl transfer appears to occur through the hole underneath the arch. The invariant residues Asn36 and Glu86 are implicated in the catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 1P4M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure | + | 1P4M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=FMN:'>FMN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. This structure supersedes the now removed PDB entry 1NBG. Active as [http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P4M OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Riboflavin kinase]] | [[Category: Riboflavin kinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Grishin, N | + | [[Category: Grishin, N V.]] |
[[Category: Karthikeyan, S.]] | [[Category: Karthikeyan, S.]] | ||
[[Category: Mseeh, F.]] | [[Category: Mseeh, F.]] | ||
| - | [[Category: Osterman, A | + | [[Category: Osterman, A L.]] |
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
[[Category: Zhou, Q.]] | [[Category: Zhou, Q.]] | ||
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[[Category: riboflavin kinase]] | [[Category: riboflavin kinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:06 2008'' |
Revision as of 12:25, 21 February 2008
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CRYSTAL STRUCTURE OF RIBOFLAVIN KINASE
Overview
Riboflavin kinase (RFK) is an essential enzyme catalyzing the phosphorylation of riboflavin (vitamin B(2)) to form FMN, an obligatory step in vitamin B(2) utilization and flavin cofactor synthesis. The structure of human RFK revealed a six-stranded antiparallel beta barrel core structurally similar to the riboflavin synthase/ferredoxin reductase FAD binding domain fold. The binding site of an intrinsically bound MgADP defines a novel nucleotide binding motif that encompasses a loop, a 3(10) helix, and a reverse turn followed by a short beta strand. This active site loop forms an arch with ATP and riboflavin binding at the opposite side and the phosphoryl transfer appears to occur through the hole underneath the arch. The invariant residues Asn36 and Glu86 are implicated in the catalysis.
About this Structure
1P4M is a Single protein structure of sequence from Homo sapiens with , and as ligands. This structure supersedes the now removed PDB entry 1NBG. Active as Riboflavin kinase, with EC number 2.7.1.26 Full crystallographic information is available from OCA.
Reference
Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch., Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H, Structure. 2003 Mar;11(3):265-73. PMID:12623014
Page seeded by OCA on Thu Feb 21 14:25:06 2008
Categories: Homo sapiens | Riboflavin kinase | Single protein | Grishin, N V. | Karthikeyan, S. | Mseeh, F. | Osterman, A L. | Zhang, H. | Zhou, Q. | ADP | FMN | MG | Beta barrel | Flavin mononucleotide
