1p65
From Proteopedia
(New page: 200px<br /><applet load="1p65" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p65, resolution 2.60Å" /> '''Crystal structure of...) |
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| - | [[Image:1p65.jpg|left|200px]]<br /><applet load="1p65" size=" | + | [[Image:1p65.jpg|left|200px]]<br /><applet load="1p65" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p65, resolution 2.60Å" /> | caption="1p65, resolution 2.60Å" /> | ||
'''Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)'''<br /> | '''Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Porcine reproductive and respiratory syndrome virus (PRRSV) is an | + | Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus of the Arteriviridae family, genomically related to the coronaviruses. PRRSV is the causative agent of both severe and persistent respiratory disease and reproductive failure in pigs worldwide. The PRRSV virion contains a core made of the 123 amino acid nucleocapsid (N) protein, a product of the ORF7 gene. We have determined the crystal structure of the capsid-forming domain of N. The structure was solved to 2.6 A resolution by SAD methods using the anomalous signal from sulfur. The N protein exists in the crystal as a tight dimer forming a four-stranded beta sheet floor superposed by two long alpha helices and flanked by two N- and two C-terminal alpha helices. The structure of N represents a new class of viral capsid-forming domains, distinctly different from those of other known enveloped viruses, but reminiscent of the coat protein of bacteriophage MS2. |
==About this Structure== | ==About this Structure== | ||
| - | 1P65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Porcine_respiratory_and_reproductive_syndrome_virus Porcine respiratory and reproductive syndrome virus]. Full crystallographic information is available from [http:// | + | 1P65 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Porcine_respiratory_and_reproductive_syndrome_virus Porcine respiratory and reproductive syndrome virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P65 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Porcine respiratory and reproductive syndrome virus]] | [[Category: Porcine respiratory and reproductive syndrome virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Doan, D | + | [[Category: Doan, D N.P.]] |
[[Category: Dokland, T.]] | [[Category: Dokland, T.]] | ||
[[Category: nucleocapsid]] | [[Category: nucleocapsid]] | ||
[[Category: virus]] | [[Category: virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:31 2008'' |
Revision as of 12:25, 21 February 2008
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Crystal structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus (PRRSV)
Overview
Porcine reproductive and respiratory syndrome virus (PRRSV) is an enveloped RNA virus of the Arteriviridae family, genomically related to the coronaviruses. PRRSV is the causative agent of both severe and persistent respiratory disease and reproductive failure in pigs worldwide. The PRRSV virion contains a core made of the 123 amino acid nucleocapsid (N) protein, a product of the ORF7 gene. We have determined the crystal structure of the capsid-forming domain of N. The structure was solved to 2.6 A resolution by SAD methods using the anomalous signal from sulfur. The N protein exists in the crystal as a tight dimer forming a four-stranded beta sheet floor superposed by two long alpha helices and flanked by two N- and two C-terminal alpha helices. The structure of N represents a new class of viral capsid-forming domains, distinctly different from those of other known enveloped viruses, but reminiscent of the coat protein of bacteriophage MS2.
About this Structure
1P65 is a Single protein structure of sequence from Porcine respiratory and reproductive syndrome virus. Full crystallographic information is available from OCA.
Reference
Structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus., Doan DN, Dokland T, Structure. 2003 Nov;11(11):1445-51. PMID:14604534
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