1p71
From Proteopedia
(New page: 200px<br /><applet load="1p71" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p71, resolution 1.90Å" /> '''Anabaena HU-DNA corc...) |
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| - | [[Image:1p71.gif|left|200px]]<br /><applet load="1p71" size=" | + | [[Image:1p71.gif|left|200px]]<br /><applet load="1p71" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p71, resolution 1.90Å" /> | caption="1p71, resolution 1.90Å" /> | ||
'''Anabaena HU-DNA corcrystal structure (TR3)'''<br /> | '''Anabaena HU-DNA corcrystal structure (TR3)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | HU and IHF are members of a family of prokaryotic proteins that interact | + | HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries. |
==About this Structure== | ==About this Structure== | ||
| - | 1P71 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http:// | + | 1P71 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P71 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Anabaena sp.]] | [[Category: Anabaena sp.]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Lemberg, K | + | [[Category: Lemberg, K M.]] |
| - | [[Category: Rice, P | + | [[Category: Rice, P A.]] |
| - | [[Category: Swinger, K | + | [[Category: Swinger, K S.]] |
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: dna bending]] | [[Category: dna bending]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:49 2008'' |
Revision as of 12:25, 21 February 2008
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Anabaena HU-DNA corcrystal structure (TR3)
Overview
HU and IHF are members of a family of prokaryotic proteins that interact with the DNA minor groove in a sequence-specific (IHF) or non-specific (HU) manner to induce and/or stabilize DNA bending. HU plays architectural roles in replication initiation, transcription regulation and site-specific recombination, and is associated with bacterial nucleoids. Cocrystal structures of Anabaena HU bound to DNA (1P71, 1P78, 1P51) reveal that while underlying proline intercalation and asymmetric charge neutralization mechanisms of DNA bending are similar for IHF and HU, HU stabilizes different DNA bend angles ( approximately 105-140 degrees ). The two bend angles within a single HU complex are not coplanar, and the resulting dihedral angle is consistent with negative supercoiling. Comparison of HU-DNA and IHF-DNA structures suggests that sharper bending is correlated with longer DNA binding sites and smaller dihedral angles. An HU-induced bend may be better modeled as a hinge, not a rigid bend. The ability to induce or stabilize varying bend angles is consistent with HU's role as an architectural cofactor in many different systems that may require differing geometries.
About this Structure
1P71 is a Single protein structure of sequence from Anabaena sp.. Full crystallographic information is available from OCA.
Reference
Flexible DNA bending in HU-DNA cocrystal structures., Swinger KK, Lemberg KM, Zhang Y, Rice PA, EMBO J. 2003 Jul 15;22(14):3749-60. PMID:12853489
Page seeded by OCA on Thu Feb 21 14:25:49 2008
