1p7n
From Proteopedia
(New page: 200px<br /><applet load="1p7n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p7n, resolution 2.60Å" /> '''Dimeric Rous Sarcoma...) |
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| - | [[Image:1p7n.jpg|left|200px]]<br /><applet load="1p7n" size=" | + | [[Image:1p7n.jpg|left|200px]]<br /><applet load="1p7n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p7n, resolution 2.60Å" /> | caption="1p7n, resolution 2.60Å" /> | ||
'''Dimeric Rous Sarcoma virus Capsid protein structure with an upstream 25-amino acid residue extension of C-terminal of Gag p10 protein'''<br /> | '''Dimeric Rous Sarcoma virus Capsid protein structure with an upstream 25-amino acid residue extension of C-terminal of Gag p10 protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the N-terminal domain (NTD) of Rous sarcoma virus (RSV) | + | The structure of the N-terminal domain (NTD) of Rous sarcoma virus (RSV) capsid protein (CA), with an upstream 25 amino acid residue extension corresponding to the C-terminal portion of the Gag p10 protein, has been determined by X-ray crystallography. Purified Gag proteins of retroviruses can assemble in vitro into virus-like particles closely resembling in vivo-assembled immature virus particles, but without a membrane. When the 25 amino acid residues upstream of CA are deleted, Gag assembles into tubular particles. The same phenotype is observed in vivo. Thus, these residues act as a "shape determinant" promoting spherical assembly, when they are present, or tubular assembly, when they are absent. We show that, unlike the NTD on its own, the extended NTD protein has no beta-hairpin loop at the N terminus of CA and that the molecule forms a dimer in which the amino-terminal extension forms the interface between monomers. Since dimerization of Gag has been inferred to be a critical step in assembly of spherical, immature Gag particles, the dimer interface may represent a structural feature that is essential in retrovirus assembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1P7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http:// | + | 1P7N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rous_sarcoma_virus Rous sarcoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7N OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rous sarcoma virus]] | [[Category: Rous sarcoma virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Francisco, A | + | [[Category: Francisco, A B.]] |
| - | [[Category: Johnson, M | + | [[Category: Johnson, M C.]] |
[[Category: Nandhagopal, N.]] | [[Category: Nandhagopal, N.]] | ||
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
| - | [[Category: Schatz, G | + | [[Category: Schatz, G W.]] |
| - | [[Category: Simpson, A | + | [[Category: Simpson, A A.]] |
| - | [[Category: Vogt, V | + | [[Category: Vogt, V M.]] |
[[Category: capsid protein]] | [[Category: capsid protein]] | ||
[[Category: gag polyprotein]] | [[Category: gag polyprotein]] | ||
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[[Category: retrovirus]] | [[Category: retrovirus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:00 2008'' |
Revision as of 12:26, 21 February 2008
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Dimeric Rous Sarcoma virus Capsid protein structure with an upstream 25-amino acid residue extension of C-terminal of Gag p10 protein
Overview
The structure of the N-terminal domain (NTD) of Rous sarcoma virus (RSV) capsid protein (CA), with an upstream 25 amino acid residue extension corresponding to the C-terminal portion of the Gag p10 protein, has been determined by X-ray crystallography. Purified Gag proteins of retroviruses can assemble in vitro into virus-like particles closely resembling in vivo-assembled immature virus particles, but without a membrane. When the 25 amino acid residues upstream of CA are deleted, Gag assembles into tubular particles. The same phenotype is observed in vivo. Thus, these residues act as a "shape determinant" promoting spherical assembly, when they are present, or tubular assembly, when they are absent. We show that, unlike the NTD on its own, the extended NTD protein has no beta-hairpin loop at the N terminus of CA and that the molecule forms a dimer in which the amino-terminal extension forms the interface between monomers. Since dimerization of Gag has been inferred to be a critical step in assembly of spherical, immature Gag particles, the dimer interface may represent a structural feature that is essential in retrovirus assembly.
About this Structure
1P7N is a Single protein structure of sequence from Rous sarcoma virus. Full crystallographic information is available from OCA.
Reference
Dimeric rous sarcoma virus capsid protein structure relevant to immature Gag assembly., Nandhagopal N, Simpson AA, Johnson MC, Francisco AB, Schatz GW, Rossmann MG, Vogt VM, J Mol Biol. 2004 Jan 2;335(1):275-82. PMID:14659756
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