1p86
From Proteopedia
(New page: 200px<br /><applet load="1p86" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p86" /> '''Real space refined coordinates of the 50S su...) |
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caption="1p86" /> | caption="1p86" /> | ||
'''Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome'''<br /> | '''Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cryo-EM density maps showing the 70S ribosome of E. coli in two different | + | Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation. |
==About this Structure== | ==About this Structure== | ||
| - | 1P86 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1P86 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P86 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Agrawal, R | + | [[Category: Agrawal, R K.]] |
| - | [[Category: Chapman, M | + | [[Category: Chapman, M S.]] |
[[Category: Eswar, N.]] | [[Category: Eswar, N.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Gao, H.]] | [[Category: Gao, H.]] | ||
| - | [[Category: Harvey, S | + | [[Category: Harvey, S T.]] |
[[Category: Korostelev, A]] | [[Category: Korostelev, A]] | ||
| - | [[Category: Roey, P | + | [[Category: Roey, P Van.]] |
[[Category: Sali, A.]] | [[Category: Sali, A.]] | ||
[[Category: Sengupta, J.]] | [[Category: Sengupta, J.]] | ||
| - | [[Category: Stagg, S | + | [[Category: Stagg, S M.]] |
[[Category: Valle, M.]] | [[Category: Valle, M.]] | ||
| - | [[Category: ef-g | + | [[Category: ef-g gtp-bound state]] |
[[Category: ratchet-like movement]] | [[Category: ratchet-like movement]] | ||
[[Category: real-space refinement]] | [[Category: real-space refinement]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:09 2008'' |
Revision as of 12:26, 21 February 2008
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Real space refined coordinates of the 50S subunit fitted into the low resolution cryo-EM map of the initiation-like state of E. coli 70S ribosome
Overview
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.
About this Structure
1P86 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Study of the structural dynamics of the E coli 70S ribosome using real-space refinement., Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J, Cell. 2003 Jun 13;113(6):789-801. PMID:12809609
Page seeded by OCA on Thu Feb 21 14:26:09 2008
