1p8g
From Proteopedia
(New page: 200px<br /><applet load="1p8g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p8g" /> '''The solution structure of apo CopZ from Baci...) |
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| - | [[Image:1p8g.jpg|left|200px]]<br /><applet load="1p8g" size=" | + | [[Image:1p8g.jpg|left|200px]]<br /><applet load="1p8g" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''The solution structure of apo CopZ from Bacillus subtilis'''<br /> | '''The solution structure of apo CopZ from Bacillus subtilis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of apo CopZ from Bacillus subtilis has been | + | The solution structure of apo CopZ from Bacillus subtilis has been determined with the aim of investigating the changes in the hydrophobic interactions around the M-X-C-X-X-C copper(I) binding motif upon metal binding. The methionine of this motif (Met 11 in CopZ) points toward the solvent in apo CopZ, whereas its sulfur atom is close to the metal ion in the metal-loaded protein, though probably not at binding distance. This change is associated with the weakening of the interaction between Leu 37 and Cys 16, present in the apo form, and the formation of an interaction between Met 11 and Tyr 65. Loops 1, 3, and 5 are affected by metal binding. Comparison with the structure of other homologous proteins confirms that often metal binding affects a hydrophobic patch around the metal site, possibly for optimizing and tuning the hydrophobic interactions with the partners. It is also shown that copper(I) exchanges among apo CopZ molecules in slow exchange on the NMR time scale, whereas it is known that such exchange between partner molecules (i.e., metallochaperones and metal pumps) is fast. |
==About this Structure== | ==About this Structure== | ||
| - | 1P8G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http:// | + | 1P8G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P8G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Banci, L.]] | [[Category: Banci, L.]] | ||
[[Category: Bertini, I.]] | [[Category: Bertini, I.]] | ||
| - | [[Category: Conte, R | + | [[Category: Conte, R Del.]] |
[[Category: beta-alpha-beta-beta-alpha-beta secondary structure]] | [[Category: beta-alpha-beta-beta-alpha-beta secondary structure]] | ||
[[Category: copper chaperone]] | [[Category: copper chaperone]] | ||
[[Category: m-x-c-x-x-c motif]] | [[Category: m-x-c-x-x-c motif]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:15 2008'' |
Revision as of 12:26, 21 February 2008
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The solution structure of apo CopZ from Bacillus subtilis
Overview
The solution structure of apo CopZ from Bacillus subtilis has been determined with the aim of investigating the changes in the hydrophobic interactions around the M-X-C-X-X-C copper(I) binding motif upon metal binding. The methionine of this motif (Met 11 in CopZ) points toward the solvent in apo CopZ, whereas its sulfur atom is close to the metal ion in the metal-loaded protein, though probably not at binding distance. This change is associated with the weakening of the interaction between Leu 37 and Cys 16, present in the apo form, and the formation of an interaction between Met 11 and Tyr 65. Loops 1, 3, and 5 are affected by metal binding. Comparison with the structure of other homologous proteins confirms that often metal binding affects a hydrophobic patch around the metal site, possibly for optimizing and tuning the hydrophobic interactions with the partners. It is also shown that copper(I) exchanges among apo CopZ molecules in slow exchange on the NMR time scale, whereas it is known that such exchange between partner molecules (i.e., metallochaperones and metal pumps) is fast.
About this Structure
1P8G is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Solution structure of apo CopZ from Bacillus subtilis: further analysis of the changes associated with the presence of copper., Banci L, Bertini I, Del Conte R, Biochemistry. 2003 Nov 25;42(46):13422-8. PMID:14621987
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