1p8k
From Proteopedia
(New page: 200px<br /><applet load="1p8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p8k, resolution 2.6Å" /> '''The structure and DNA...) |
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| - | [[Image:1p8k.gif|left|200px]]<br /><applet load="1p8k" size=" | + | [[Image:1p8k.gif|left|200px]]<br /><applet load="1p8k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p8k, resolution 2.6Å" /> | caption="1p8k, resolution 2.6Å" /> | ||
'''The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor'''<br /> | '''The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We determined the crystal structure of a bifunctional group I intron | + | We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 A resolution. The structure demonstrates the remarkable structural conservation of the beta-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant from the DNA-binding surface, that might be involved in RNA binding. A point mutation that introduces a single negative charge in this region uncouples the maturase and endonuclease functions of the protein, inhibiting RNA binding and splicing while maintaining DNA binding and cleavage. |
==About this Structure== | ==About this Structure== | ||
| - | 1P8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1P8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P8K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bolduc, J | + | [[Category: Bolduc, J M.]] |
| - | [[Category: Stoddard, B | + | [[Category: Stoddard, B L.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: hydrolase/dna]] | [[Category: hydrolase/dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:17 2008'' |
Revision as of 12:26, 21 February 2008
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The structure and DNA recognition of a bifunctional homing endonuclease and group I intron splicing factor
Overview
We determined the crystal structure of a bifunctional group I intron splicing factor and homing endonuclease, termed the I-AniI maturase, in complex with its DNA target at 2.6 A resolution. The structure demonstrates the remarkable structural conservation of the beta-sheet DNA-binding motif between highly divergent enzyme subfamilies. DNA recognition by I-AniI was further studied using nucleoside deletion and DMS modification interference analyses. Correlation of these results with the crystal structure provides information on the relative importance of individual nucleotide contacts for DNA recognition. Alignment and modeling of two homologous maturases reveals conserved basic surface residues, distant from the DNA-binding surface, that might be involved in RNA binding. A point mutation that introduces a single negative charge in this region uncouples the maturase and endonuclease functions of the protein, inhibiting RNA binding and splicing while maintaining DNA binding and cleavage.
About this Structure
1P8K is a Single protein structure of sequence from Emericella nidulans with as ligand. Full crystallographic information is available from OCA.
Reference
Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor., Bolduc JM, Spiegel PC, Chatterjee P, Brady KL, Downing ME, Caprara MG, Waring RB, Stoddard BL, Genes Dev. 2003 Dec 1;17(23):2875-88. Epub 2003 Nov 21. PMID:14633971
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