1p9h
From Proteopedia
(New page: 200px<br /><applet load="1p9h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9h, resolution 1.55Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1p9h.gif|left|200px]]<br /><applet load="1p9h" size=" | + | [[Image:1p9h.gif|left|200px]]<br /><applet load="1p9h" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1p9h, resolution 1.55Å" /> | caption="1p9h, resolution 1.55Å" /> | ||
'''CRYSTAL STRUCTURE OF THE COLLAGEN-BINDING DOMAIN OF YERSINIA ADHESIN YadA'''<br /> | '''CRYSTAL STRUCTURE OF THE COLLAGEN-BINDING DOMAIN OF YERSINIA ADHESIN YadA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the recombinant collagen-binding domain of | + | The crystal structure of the recombinant collagen-binding domain of Yersinia adhesin YadA from Yersinia enterocolitica serotype O:3 was solved at 1.55 A resolution. The trimeric structure is composed of head and neck regions, and the collagen binding head region is a novel nine-coiled left-handed parallel beta-roll. Before the beta-roll, the polypeptide loops from one monomer to the rest, and after the beta-roll the neck region does the same, making the transition from the globular head region to the narrower stalk domain. This creates an intrinsically stable 'lock nut' structure. The trimeric form of YadA is required for collagen binding, and mutagenesis of its surface residues allowed identification of a putative collagen-binding surface. Furthermore, a new structure-sequence motif for YadA beta-roll was used to identify putative YadA-head-like domains in a variety of human and plant pathogens. Such domains may therefore be a common bacterial strategy for avoiding host response. |
==About this Structure== | ==About this Structure== | ||
| - | 1P9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http:// | + | 1P9H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Yersinia enterocolitica]] | [[Category: Yersinia enterocolitica]] | ||
[[Category: Goldman, A.]] | [[Category: Goldman, A.]] | ||
| - | [[Category: Merckel, M | + | [[Category: Merckel, M C.]] |
[[Category: Nummelin, H.]] | [[Category: Nummelin, H.]] | ||
[[Category: Skurnik, M.]] | [[Category: Skurnik, M.]] | ||
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[[Category: left-handed beta-roll]] | [[Category: left-handed beta-roll]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:34 2008'' |
Revision as of 12:26, 21 February 2008
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CRYSTAL STRUCTURE OF THE COLLAGEN-BINDING DOMAIN OF YERSINIA ADHESIN YadA
Overview
The crystal structure of the recombinant collagen-binding domain of Yersinia adhesin YadA from Yersinia enterocolitica serotype O:3 was solved at 1.55 A resolution. The trimeric structure is composed of head and neck regions, and the collagen binding head region is a novel nine-coiled left-handed parallel beta-roll. Before the beta-roll, the polypeptide loops from one monomer to the rest, and after the beta-roll the neck region does the same, making the transition from the globular head region to the narrower stalk domain. This creates an intrinsically stable 'lock nut' structure. The trimeric form of YadA is required for collagen binding, and mutagenesis of its surface residues allowed identification of a putative collagen-binding surface. Furthermore, a new structure-sequence motif for YadA beta-roll was used to identify putative YadA-head-like domains in a variety of human and plant pathogens. Such domains may therefore be a common bacterial strategy for avoiding host response.
About this Structure
1P9H is a Single protein structure of sequence from Yersinia enterocolitica. Full crystallographic information is available from OCA.
Reference
The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll., Nummelin H, Merckel MC, Leo JC, Lankinen H, Skurnik M, Goldman A, EMBO J. 2004 Feb 25;23(4):701-11. Epub 2004 Feb 5. PMID:14765110
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