1pbv
From Proteopedia
(New page: 200px<br /> <applet load="1pbv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pbv, resolution 2.00Å" /> '''SEC7 DOMAIN OF THE ...) |
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caption="1pbv, resolution 2.00Å" /> | caption="1pbv, resolution 2.00Å" /> | ||
'''SEC7 DOMAIN OF THE EXCHANGE FACTOR ARNO'''<br /> | '''SEC7 DOMAIN OF THE EXCHANGE FACTOR ARNO'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Small G proteins switch from a resting, GDP-bound state to an active, GTP-bound state. As spontaneous GDP release is slow, guanine-nucleotide-exchange factors (GEFs) are required to promote fast | + | Small G proteins switch from a resting, GDP-bound state to an active, GTP-bound state. As spontaneous GDP release is slow, guanine-nucleotide-exchange factors (GEFs) are required to promote fast activation of small G proteins through replacement of GDP with GTP in vivo. Families of GEFs with no sequence similarity to other GEF families have now been assigned to most families of small G proteins. In the case of the small G protein Arf1, the exchange of bound GDP for GTP promotes the coating of secretory vesicles in Golgi traffic. An exchange factor for human Arf1, ARNO, and two closely related proteins, named cytohesin 1 and GPS1, have been identified. These three proteins are modular proteins with an amino-terminal coiled-coil, a central Sec7-like domain and a carboxy-terminal pleckstrin homology domain. The Sec7 domain contains the exchange-factor activity. It was first found in Sec7, a yeast protein involved in secretion, and is present in several other proteins, including the yeast exchange factors for Arf, Geal and Gea2. Here we report the crystal structure of the Sec7 domain of human ARNO at 2 A resolution and the identification of the site of interaction of ARNO with Arf. |
==About this Structure== | ==About this Structure== | ||
| - | 1PBV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1PBV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PBV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Antonny, B.]] | [[Category: Antonny, B.]] | ||
[[Category: Beraud-Dufour, S.]] | [[Category: Beraud-Dufour, S.]] | ||
| - | [[Category: Bras, G | + | [[Category: Bras, G Le.]] |
[[Category: Chardin, P.]] | [[Category: Chardin, P.]] | ||
[[Category: Cherfils, J.]] | [[Category: Cherfils, J.]] | ||
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[[Category: sec7]] | [[Category: sec7]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:14 2008'' |
Revision as of 12:27, 21 February 2008
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SEC7 DOMAIN OF THE EXCHANGE FACTOR ARNO
Overview
Small G proteins switch from a resting, GDP-bound state to an active, GTP-bound state. As spontaneous GDP release is slow, guanine-nucleotide-exchange factors (GEFs) are required to promote fast activation of small G proteins through replacement of GDP with GTP in vivo. Families of GEFs with no sequence similarity to other GEF families have now been assigned to most families of small G proteins. In the case of the small G protein Arf1, the exchange of bound GDP for GTP promotes the coating of secretory vesicles in Golgi traffic. An exchange factor for human Arf1, ARNO, and two closely related proteins, named cytohesin 1 and GPS1, have been identified. These three proteins are modular proteins with an amino-terminal coiled-coil, a central Sec7-like domain and a carboxy-terminal pleckstrin homology domain. The Sec7 domain contains the exchange-factor activity. It was first found in Sec7, a yeast protein involved in secretion, and is present in several other proteins, including the yeast exchange factors for Arf, Geal and Gea2. Here we report the crystal structure of the Sec7 domain of human ARNO at 2 A resolution and the identification of the site of interaction of ARNO with Arf.
About this Structure
1PBV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Sec7 domain of the Arf exchange factor ARNO., Cherfils J, Menetrey J, Mathieu M, Le Bras G, Robineau S, Beraud-Dufour S, Antonny B, Chardin P, Nature. 1998 Mar 5;392(6671):101-5. PMID:9510256
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