1pc5
From Proteopedia
(New page: 200px<br /><applet load="1pc5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pc5, resolution 1.80Å" /> '''Crystal Structure of...) |
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| - | [[Image:1pc5.gif|left|200px]]<br /><applet load="1pc5" size=" | + | [[Image:1pc5.gif|left|200px]]<br /><applet load="1pc5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pc5, resolution 1.80Å" /> | caption="1pc5, resolution 1.80Å" /> | ||
'''Crystal Structure of the P50G Mutant of Ferredoxin I at 1.8 A Resolution'''<br /> | '''Crystal Structure of the P50G Mutant of Ferredoxin I at 1.8 A Resolution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 7Fe ferredoxin from Azotobacter vinelandii (AvFdI) contains a | + | The 7Fe ferredoxin from Azotobacter vinelandii (AvFdI) contains a [3Fe-4S](+/0) cluster that binds a single proton in its reduced level. Although the cluster is buried, and therefore inaccessible to solvent, proton transfer from solvent to the cluster is fast. The kinetics and energetics of the coupled electron-proton transfer reaction at the cluster have been analyzed in detail by protein-film voltammetry, to reveal that proton transfer is mediated by the mobile carboxylate of an adjacent surface residue, aspartate-15, the pK of which is sensitive to the charge on the cluster. This paper examines the role of a nearby proline residue, proline-50, in proton transfer and its coupling to electron transfer. In the P50A and P50G mutants, a water molecule has entered the cluster binding region; it is hydrogen bonded to the backbone amide of residue-50 and to the Asp-15 carboxylate, and it is approximately 4 A from the closest sulfur atom of the cluster. Despite the water molecule linking the cluster more directly to the solvent, proton transfer is not accelerated. A detailed analysis reveals that Asp-15 remains a central part of the mechanism. However, the electrostatic coupling between cluster and carboxylate is almost completely quenched, so that cluster reduction no longer induces such a favorable shift in the carboxylate pK, and protonation of the base no longer induces a significant shift in the pK of the cluster. The electrostatic coupling is crucial for maintaining the efficiency of proton transfer both to and from the cluster, over a range of pH values. |
==About this Structure== | ==About this Structure== | ||
| - | 1PC5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with SF4 and F3S as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1PC5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=F3S:'>F3S</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PC5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Azotobacter vinelandii]] | [[Category: Azotobacter vinelandii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Armstrong, F | + | [[Category: Armstrong, F A.]] |
| - | [[Category: Burgess, B | + | [[Category: Burgess, B K.]] |
[[Category: Camba, R.]] | [[Category: Camba, R.]] | ||
[[Category: Chen, K.]] | [[Category: Chen, K.]] | ||
[[Category: Hirst, J.]] | [[Category: Hirst, J.]] | ||
| - | [[Category: Hunsicker-Wang, L | + | [[Category: Hunsicker-Wang, L M.]] |
| - | [[Category: Jung, Y | + | [[Category: Jung, Y S.]] |
| - | [[Category: Stout, C | + | [[Category: Stout, C D.]] |
[[Category: F3S]] | [[Category: F3S]] | ||
[[Category: SF4]] | [[Category: SF4]] | ||
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[[Category: mutant]] | [[Category: mutant]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:24 2008'' |
Revision as of 12:27, 21 February 2008
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Crystal Structure of the P50G Mutant of Ferredoxin I at 1.8 A Resolution
Overview
The 7Fe ferredoxin from Azotobacter vinelandii (AvFdI) contains a [3Fe-4S](+/0) cluster that binds a single proton in its reduced level. Although the cluster is buried, and therefore inaccessible to solvent, proton transfer from solvent to the cluster is fast. The kinetics and energetics of the coupled electron-proton transfer reaction at the cluster have been analyzed in detail by protein-film voltammetry, to reveal that proton transfer is mediated by the mobile carboxylate of an adjacent surface residue, aspartate-15, the pK of which is sensitive to the charge on the cluster. This paper examines the role of a nearby proline residue, proline-50, in proton transfer and its coupling to electron transfer. In the P50A and P50G mutants, a water molecule has entered the cluster binding region; it is hydrogen bonded to the backbone amide of residue-50 and to the Asp-15 carboxylate, and it is approximately 4 A from the closest sulfur atom of the cluster. Despite the water molecule linking the cluster more directly to the solvent, proton transfer is not accelerated. A detailed analysis reveals that Asp-15 remains a central part of the mechanism. However, the electrostatic coupling between cluster and carboxylate is almost completely quenched, so that cluster reduction no longer induces such a favorable shift in the carboxylate pK, and protonation of the base no longer induces a significant shift in the pK of the cluster. The electrostatic coupling is crucial for maintaining the efficiency of proton transfer both to and from the cluster, over a range of pH values.
About this Structure
1PC5 is a Single protein structure of sequence from Azotobacter vinelandii with and as ligands. Full crystallographic information is available from OCA.
Reference
Mechanisms of redox-coupled proton transfer in proteins: role of the proximal proline in reactions of the [3Fe-4S] cluster in Azotobacter vinelandii ferredoxin I., Camba R, Jung YS, Hunsicker-Wang LM, Burgess BK, Stout CD, Hirst J, Armstrong FA, Biochemistry. 2003 Sep 16;42(36):10589-99. PMID:12962482
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