1pd3
From Proteopedia
(New page: 200px<br /><applet load="1pd3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pd3, resolution 2.60Å" /> '''Influenza A NEP M1-b...) |
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| - | [[Image:1pd3.gif|left|200px]]<br /><applet load="1pd3" size=" | + | [[Image:1pd3.gif|left|200px]]<br /><applet load="1pd3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pd3, resolution 2.60Å" /> | caption="1pd3, resolution 2.60Å" /> | ||
'''Influenza A NEP M1-binding domain'''<br /> | '''Influenza A NEP M1-binding domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During influenza virus infection, viral ribonucleoproteins (vRNPs) are | + | During influenza virus infection, viral ribonucleoproteins (vRNPs) are replicated in the nucleus and must be exported to the cytoplasm before assembling into mature viral particles. Nuclear export is mediated by the cellular protein Crm1 and putatively by the viral protein NEP/NS2. Proteolytic cleavage of NEP defines an N-terminal domain which mediates RanGTP-dependent binding to Crm1 and a C-terminal domain which binds to the viral matrix protein M1. The 2.6 A crystal structure of the C-terminal domain reveals an amphipathic helical hairpin which dimerizes as a four-helix bundle. The NEP-M1 interaction involves two critical epitopes: an exposed tryptophan (Trp78) surrounded by a cluster of glutamate residues on NEP, and the basic nuclear localization signal (NLS) of M1. Implications for vRNP export are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1PD3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http:// | + | 1PD3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PD3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: nep/ns2]] | [[Category: nep/ns2]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:35 2008'' |
Revision as of 12:27, 21 February 2008
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Influenza A NEP M1-binding domain
Overview
During influenza virus infection, viral ribonucleoproteins (vRNPs) are replicated in the nucleus and must be exported to the cytoplasm before assembling into mature viral particles. Nuclear export is mediated by the cellular protein Crm1 and putatively by the viral protein NEP/NS2. Proteolytic cleavage of NEP defines an N-terminal domain which mediates RanGTP-dependent binding to Crm1 and a C-terminal domain which binds to the viral matrix protein M1. The 2.6 A crystal structure of the C-terminal domain reveals an amphipathic helical hairpin which dimerizes as a four-helix bundle. The NEP-M1 interaction involves two critical epitopes: an exposed tryptophan (Trp78) surrounded by a cluster of glutamate residues on NEP, and the basic nuclear localization signal (NLS) of M1. Implications for vRNP export are discussed.
About this Structure
1PD3 is a Single protein structure of sequence from Influenza a virus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2)., Akarsu H, Burmeister WP, Petosa C, Petit I, Muller CW, Ruigrok RW, Baudin F, EMBO J. 2003 Sep 15;22(18):4646-55. PMID:12970177
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