1pdm
From Proteopedia
(New page: 200px<br /><applet load="1pdm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pdm" /> '''Fitting of gp8 structure into the cryoEM rec...) |
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'''Fitting of gp8 structure into the cryoEM reconstruction of the bacteriophage T4 baseplate'''<br /> | '''Fitting of gp8 structure into the cryoEM reconstruction of the bacteriophage T4 baseplate'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The baseplate of bacteriophage T4 is a multiprotein molecular machine that | + | The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection. |
==About this Structure== | ==About this Structure== | ||
| - | 1PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http:// | + | 1PDM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PDM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Arisaka, F.]] | [[Category: Arisaka, F.]] | ||
| - | [[Category: Chipman, P | + | [[Category: Chipman, P R.]] |
[[Category: Kanamaru, S.]] | [[Category: Kanamaru, S.]] | ||
| - | [[Category: Kostyuchenko, V | + | [[Category: Kostyuchenko, V A.]] |
| - | [[Category: Leiman, P | + | [[Category: Leiman, P G.]] |
| - | [[Category: Mesyanzhinov, V | + | [[Category: Mesyanzhinov, V V.]] |
| - | [[Category: Raaij, M | + | [[Category: Raaij, M J.van.]] |
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
[[Category: structural protein]] | [[Category: structural protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:27:40 2008'' |
Revision as of 12:27, 21 February 2008
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Fitting of gp8 structure into the cryoEM reconstruction of the bacteriophage T4 baseplate
Overview
The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.
About this Structure
1PDM is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of bacteriophage T4 baseplate., Kostyuchenko VA, Leiman PG, Chipman PR, Kanamaru S, van Raaij MJ, Arisaka F, Mesyanzhinov VV, Rossmann MG, Nat Struct Biol. 2003 Sep;10(9):688-93. Epub 2003 Aug 17. PMID:12923574
Page seeded by OCA on Thu Feb 21 14:27:40 2008
