1pfi
From Proteopedia
(New page: 200px<br /><applet load="1pfi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pfi, resolution 3.0Å" /> '''PF1 VIRUS STRUCTURE: ...) |
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| - | [[Image:1pfi.gif|left|200px]]<br /><applet load="1pfi" size=" | + | [[Image:1pfi.gif|left|200px]]<br /><applet load="1pfi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pfi, resolution 3.0Å" /> | caption="1pfi, resolution 3.0Å" /> | ||
'''PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES'''<br /> | '''PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The helical path of the DNA in filamentous bacteriophage Pf1 was deduced | + | The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices. |
==About this Structure== | ==About this Structure== | ||
| - | 1PFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1] with C as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PFI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1] with <scene name='pdbligand=C:'>C</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PFI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas phage pf1]] | [[Category: Pseudomonas phage pf1]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Day, L | + | [[Category: Day, L A.]] |
| - | [[Category: Liu, D | + | [[Category: Liu, D J.]] |
[[Category: C]] | [[Category: C]] | ||
[[Category: complex(viral coat protein/dna)]] | [[Category: complex(viral coat protein/dna)]] | ||
[[Category: helical virus]] | [[Category: helical virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:28:13 2008'' |
Revision as of 12:28, 21 February 2008
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PF1 VIRUS STRUCTURE: HELICAL COAT PROTEIN AND DNA WITH PARAXIAL PHOSPHATES
Overview
The helical path of the DNA in filamentous bacteriophage Pf1 was deduced from different kinds of existing structural information, including results from x-ray fiber diffraction. The DNA has the same pitch, 16 angstroms, as the surrounding helix of protein subunits; the rise and rotation per nucleotides are 6.1 angstroms and 132 degrees, respectively; and the phosphates are 2.5 angstroms from the axis. The DNA in Pf1 is, therefore, the most extended and twisted DNA structure known. On the basis of the DNA structure and extensive additional information about the protein, a model of the virion is proposed. In the model, the DNA bases reach out, into the protein, and the lysine and arginine side chains reach in, between the DNA bases, to stabilize the paraxial phosphate charges; the conformation of the protein subunit is a combination of alpha and 3(10) helices.
About this Structure
1PFI is a Single protein structure of sequence from Pseudomonas phage pf1 with as ligand. Full crystallographic information is available from OCA.
Reference
Pf1 virus structure: helical coat protein and DNA with paraxial phosphates., Liu DJ, Day LA, Science. 1994 Jul 29;265(5172):671-4. PMID:8036516
Page seeded by OCA on Thu Feb 21 14:28:13 2008
