1pjy
From Proteopedia
(New page: 200px<br /> <applet load="1pjy" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pjy" /> '''Solution structure of the HIV-1 frameshift ...) |
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'''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''<br /> | '''Solution structure of the HIV-1 frameshift inducing stem-loop RNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The translation of reverse transcriptase and other essential viral | + | The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae. |
==About this Structure== | ==About this Structure== | ||
| - | 1PJY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1PJY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJY OCA]. |
==Reference== | ==Reference== | ||
Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12888491 12888491] | Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12888491 12888491] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Butcher, S | + | [[Category: Butcher, S E.]] |
| - | [[Category: Staple, D | + | [[Category: Staple, D W.]] |
[[Category: frameshift]] | [[Category: frameshift]] | ||
[[Category: hiv]] | [[Category: hiv]] | ||
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[[Category: tetraloop]] | [[Category: tetraloop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:34 2008'' |
Revision as of 12:29, 21 February 2008
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Solution structure of the HIV-1 frameshift inducing stem-loop RNA
Overview
The translation of reverse transcriptase and other essential viral proteins from the HIV-1 Pol mRNA requires a programmed -1 ribosomal frameshift. This frameshift is induced by two highly conserved elements within the HIV-1 mRNA: a slippery sequence comprised of a UUUUUUA heptamer, and a downstream stem-loop structure. We have determined the structure of the HIV-1 frameshift inducing RNA stem-loop, using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The 22 nucleotide RNA solution structure [root mean squared deviation (r.m.s.d.) = 1.2 A] was determined from 475 nuclear Overhauser effect (NOE)-derived distance restrains, 20 residual dipolar couplings and direct detection of hydrogen bonds via scalar couplings. We find that the frameshift inducing stem-loop is an A-form helix capped by a structured ACAA tetraloop. The ACAA tetraloop is stabilized by an equilateral 5' and 3' stacking pattern, a sheared A-A pair and a cross-strand hydrogen bond. Unexpectedly, the ACAA tetraloop structure is nearly identical to a known tetraloop fold, previously identified in the RNase III recognition site from Saccharomyces cerevisiae.
About this Structure
1PJY is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of the HIV-1 frameshift inducing stem-loop RNA., Staple DW, Butcher SE, Nucleic Acids Res. 2003 Aug 1;31(15):4326-31. PMID:12888491
Page seeded by OCA on Thu Feb 21 14:29:34 2008
Categories: Protein complex | Butcher, S E. | Staple, D W. | Frameshift | Hiv | Nmr | Rna structure | Tetraloop
